中文摘要：产毒串珠镰刀菌工业和农业用玉米的质量和效用。由于病原体与其宿主之间亲密的内生生存策略，使得化学控制变得复杂。本研究发现，漠海威芽孢杆菌RRC101（一种玉米内生菌）接种玉米后能够降低串珠镰刀菌病害的严重程度以及伏马菌素的累积。研究数据表明，抗生素和毒素生产是  玉米内生真菌复杂生化作用的组成部分，一种是致病性的，一种是有益的。
外文摘要：The mycotoxigenic pathogen Fusarium verticillioides threatens the quality and utility of maize across industrial and agricultural purposes. Chemical control is complicated by the intimate endophytic lifestyle of the pathogen with its host. Bacillus mojavensis RRC101, a maize-endophytic bacterium, has been observed to reduce F. verticillioides disease severity and fumonisin accumulation when coinoculated to maize. Genome sequencing and annotation identified a number of biocontrol-relevant pathways in RRC101. Biochemical assays confirmed the presence and activity of surfactin- and fengycin-type lipopeptides, with fengycins responsible for antifungal activity against F. verticillioides. This antagonism manifests as inhibition of filamentous growth, with microscopy revealing hyphal distortions, vacuolization, and lysis. F. verticillioides secondary metabolism also responds to antagonism, with lipopeptide challenge inducing greater fumonisin production and, in the case of fengycins, eliciting pigment accumulation at sites of inhibition. Together, these data suggest that antibiotic and toxin production are components of a complex biochemical interaction among maize endophytes, one pathogenic and one beneficial.
外文关键词：BACTERIAL ENDOPHYTES; SECONDARY METABOLISM; FUMONISIN PRODUCTION; DEFENSE RESPONSES; GENE CLUSTERS; PECTATE LYASE; SUBTILIS; SURFACTIN; FENGYCIN; MAIZE
作者：Blacutt, A. A.; Mitchell, T. R.; Bacon, C. W.; Gold, S. E.
作者单位：USDA ARS，USA
期刊名称：MOLECULAR PLANT-MICROBE INTERACTIONS
期刊影响因子：4.145
出版年份：2016
出版刊次：9
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