中文摘要:黄曲霉是地球上最丰富且广泛分布的一种真菌。黄曲霉产生的黄曲霉毒素是一种有毒的次生代谢产物,对人类和动物健康以及农作物均非常有害。本研究利用ISSR标记方法对从五种农作物和空气种分离出的30种黄曲霉的遗传多样性进行分析。该研究结果表明,ISSR技术非常适用于对不同来源的黄曲霉的遗传多样性进行特征鉴定。
外文摘要:Aspergillus flavus is one of the most abundant and widely distributed fungi on earth. A. flavus produces aflatoxins (AFs), which are toxic secondary metabolites. AFs have harmful effects on public health (humans and animals) and agricultural crops. Inter-simple sequence repeat (ISSR) markers were used to analyze the genetic diversity of 30 A. flavus isolates from five agricultural crops and air. Genetic similarity coefficients (GSC) ranged from 0.51 to 0.10 based on three ISSR markers for the isolates tested. A. flavus isolates grouped into 6, 5, and 3 clusters using the unweighted pair-group method with arithmetic average of three ISSR markers. This study suggests that ISSR biotechnology is a highly useful tool for characterizing genetic diversity of A. flavus isolated from different sources.
外文关键词:Aspergillus flavus;Inter-simple sequence repeats;Genetic diversity
作者:Mahmoud, M. A.; El-Samawaty, A. M. A.; Yassin, M. A.; Abd El-Aziz, A. R. M.
作者单位:Agr Res Ctr, Plant Pathol Res Inst,Egypt
期刊名称:GENETICS AND MOLECULAR RESEARCH
期刊影响因子:0.764
出版年份:2016
出版刊次:2
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