中文摘要：本研究开发了一种可以快速、敏感和特异性检测黄曲霉毒素B1（AFB1）的荧光检测法。利用该方法对婴儿米粉进行试验，回收率为93.0%-106.8%，这表明，该方法在食品AFB1检测中具有潜在的应用价值。
外文摘要：A fluorescent assay for the rapid, sensitive and specific detection of Aflatoxin B1 (AFB1) was developed in this study. Initially, a DNA/DNA duplex was formed between a fluorescein-labeled AFB1 aptamer and its partially complementary DNA strand containing a quencher moiety, resulting in fluorescence quenching due to the close proximity of fluorophore and quencher. Upon the addition of AFB1, an aptamer/AFB1 complex was generated to release the quencher-modified DNA strand, thus recovered the fluorescence of fluorescein and enabled quantitative detection for AFB1 by monitoring fluorescence enhancement. Under optimized conditions, this assay exhibited a linear response to AFB1 in the range of 5-100ng/mL with a detection limit down to 1.6ng/mL. Trials of this assay in infant rice cereal with satisfactory recovery in the range of 93.0%-106.8%, demonstrate that the new assay could be a potential sensing platform for AFB1 determination in food.
外文关键词：Aflatoxin B-1;Aptamer;Fluorescent;Quenching;Infant rice cereal
作者：Chen, Lu; Wen, Fang; Li, Ming; Guo, Xiaodong; Li, Songli; Zheng, Nan; Wang, Jiaqi FOOD CHEMIST
作者单位：Chinese Acad Agr Sci
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：1
点击下载：一种简单的核酸适体荧光测定法用于检测婴儿米粉中黄曲霉毒素B1