中文摘要：本研究对摩洛哥不同地区98例粗麦粉（couscous semolina）样本的多霉菌毒素污染情况进行了调查，所涉及的霉菌毒素共有22种，包括：four aflatoxins, ochratoxin A, diacetoxiscyrpenol (DAS), three fumonisins, beauvericin (BEA), deoxynivalenol (DON), 15-acetyl-deoxynivalenol (15-ADON), 3-acetyl-deoxynivalenol (3-ADON), nivalenol (NIV), sterigmatocystin (STG), zearalenone (ZEA), four enniatins, T-2 以及HT-2 toxins。结果表明，其中96个样品中至少被一种霉菌毒素污染。其中，Enniatin B (ENB), Enniatin B1 (ENB1), Enniatin A1 (ENA1) 和zearalenone (ZEA) 在样品中的发病率最高。
外文摘要：A fluorescent assay for the rapid, sensitive and specific detection of Aflatoxin B1 (AFB1) was developed in this study. Initially, a DNA/DNA duplex was formed between a fluorescein-labeled AFB1 aptamer and its partially complementary DNA strand containing a quencher moiety, resulting in fluorescence quenching due to the close proximity of fluorophore and quencher. Upon the addition of AFB1, an aptamer/AFB1 complex was generated to release the quencher-modified DNA strand, thus recovered the fluorescence of fluorescein and enabled quantitative detection for AFB1 by monitoring fluorescence enhancement. Under optimized conditions, this assay exhibited a linear response to AFB1 in the range of 5-100ng/mL with a detection limit down to 1.6ng/mL. Trials of this assay in infant rice cereal with satisfactory recovery in the range of 93.0%-106.8%, demonstrate that the new assay could be a potential sensing platform for AFB1 determination in food.
外文关键词：Couscous semolina;Multi-mycotoxin;Occurrence;Dietary intake;Morocco
作者：Zinedine, Abdellah; Fernandez-Franzon, Monica; Manes, Jordi; Manyes, Lara
作者单位：Chouaib Doukkali Univ,Morocco
期刊名称：FOOD CHEMISTRY FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：1
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