中文摘要：在商业化的转基因作物中，导入cry基因的抗虫转基因作物是一种主要品种。转cry1Ab/1Ac融合基因(GFM cry1A)和cry1Ac基因的截短片段（cry1Ac-Mon）分别是中国研究者和孟山都技术有限公司研究转基因作物的关键外源基因。本文将这两个基因均称为“GM cry1A”，用快速可视化环介导等温扩增法（LAMP）检测转基因抗虫作物中的转cry1A基因，并用LAMP方法及PCR方法分析含有或没有转基因cry1A基因的植株。研究发现，LAMP方法的灵敏度是常规PCR方法的10倍以上；与PCR方法相比，LAMP方法对转cry1A基因表现出明显的特异性。研究结果表明，对于从中国和其他发展中国家广泛种植的转基因作物中筛选转基因cry1A基因而言，LAMP方法是潜在有效的工具。
外文摘要：Among the commercial genetically modified (GM) crops, the insect-resistant GM crops are the major cultivars that cry gene is introduced into. A cry1Ab/1Ac GM fusion gene (GFM cry1A) and a GM truncated cry1Ac gene (cry1Ac-Mon) is the key foreign gene employed for construction of GM crops by China researchers and Monsanto Technology LLC respectively. Here these two genes are entitled 'GM cry1A' gene and a rapid visual loop-mediated isothermal amplification (LAMP) assay method for detection of GM cry1A in transgenic insect-resistant crops was established. The LAMP assay was performed at an optimal temperature of 65 degrees C for 60 min in the presence of a set of four specific primers recognized six distinct sequences of the GM cry1A gene. The rough detection limit to the GM cry1A in samples is as low as 0.01% (a weight ratio of transgenic insect-resistant rice/cotton to non-transgenic rice/cotton). Comparatively, the sensitivity of this LAMP method is 10 times over that of the conventional PCR method. Fifteen cultivars/events and five Bt strains with or without cry1A gene were analyzed using the LAMP method as well as PCR method. The results demonstrate that this LAMP method shows a distinct specificity to the GM cry1A gene comparing with PCR analysis. Therefore, this LAMP method will be a potential effective tool for screening the GM cry1A gene in GM crops which are widely plant in China and other developing countries. (C) 2015 Elsevier Ltd. All rights reserved.
外文关键词：Insect-resistant GM crop；GM cry1A gene；Loop-mediated isothermal amplification；PCR
作者：Peili Shen；Fengzhen Geng；Yan Yu；Yunzhe Zhang；Zhixin Wang；Zhihui Li；Wei Zhang；Changlong Shu；Yongjun Zhang；Jianxin Tan
作者单位：河北农业大学
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2016
出版刊次：4
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