中文摘要：本研究提出一种用液相色谱-串联质谱法对牛奶中15种霉菌毒素进行同时定量检测的方法。提取液为酸化乙腈，用醋酸钠洗脱。在检验过程中，对检出限(LOD)、定量限、线性、准确度、精确度、回收率、基体效应和稳定性进行了研究。15种霉菌毒素的LOD值在0.02 到10.14 ng/mL之间。回收率除伏马菌素在42.1%到64.6%之间外，其余均在82.6到94.4%之间。所有霉菌毒素，特别是deepoxy-脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮和柄曲霉素均存在25.5到96.8%的基体效应。该方法除能检测牛奶中受到普遍关注的霉菌毒素外，也能检测少有研究的伏马菌素、柄曲霉素及赭曲霉素。
外文摘要：The simultaneous quantification of 15 mycotoxins in cow milk by liquid chromatography-mass spectrometry, is presented. Extraction was performed with acidified acetonitrile, followed by a cleanup step with sodium acetate. During validation limits of detection (LOD) and quantification, linearity, precision, accuracy, recovery, matrix effect, and stability were studied. LOD values were between 0.02 and 10.14 ng/mL for aflatoxins M1, B1, B2, G1, G2, ochratoxins A and B, HT-2 and T-2 toxins, deepoxy-deoxynivalenol, zearalenone, sterigmatocystin and fumonisins B1, B2 and B3. Recovery values were between 82.6 and 94.4% for all the mycotoxins, except for fumonisins. The recovery values for fumonisins were between 42.1% and 64.6%. Matrix effect, between 25.5 and 96.8%, appeared for all of the mycotoxins, especially for deepoxy-deoxynivalenol, zearalenone and sterigmatocystin. The validated method achieves the quantification of those mycotoxins of major concern and mycotoxins that are not frequently studied in milk, such as fumonisins, sterigmatocystin or ochratoxin B.
外文关键词：Mycotoxin；Aflatoxin；Fumonisin；Ochratoxin；Milk；LC-MS/MS
作者：Flores-Flores, ME；Gonzalez-Penas, E
作者单位：Univ Navarra
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：218
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