中文摘要：本研究提出用稳定同位素稀释-液相色谱-串联质谱法检测啤酒中12种霉菌毒素，并对从德国及全球采集的61个啤酒样品进行了检测实验。12种霉菌毒素包括：DON-3-葡萄糖苷（DON-3-G），DON，3-乙酰DON（3-ADON），15-乙酰DON（15-ADON），HT-2毒素，T-2毒素，恩镰孢菌素B、B1、A1、A，白僵菌素和玉米烯酮。检测结果令人满意：日内精确度、日间精确度和回收率分别为1-5%、2-8%和72-117%。总之, 全部样品中，检测到极少量DON、DON-3-G、3-ADON和恩镰孢菌素B；没有检测到15-ADON、HT-2毒素、T-2毒素、恩镰孢菌素B1、A1、A、白僵菌素和玉米烯酮。
外文摘要：A stable isotope dilution LC-MS/MS multi-mycotoxin method was developed for 12 different Fusarium toxins including modified mycotoxins in beer (deoxynivalenol-3-glucoside, deoxynivalenol, 3-acetyl deoxynivalenol, 15-acetyl-deoxynivalenol, HT2-toxin, T2-toxin, enniatin B, B1, A1, A, beauvericin and zearalenone). As sample preparation and purification of beer a combined solid phase extraction for trichothecenes, enniatins, beauvericin and zearalenone was firstly developed. The validation of the new method gave satisfying results: intra-day and inter-day precision and recoveries were 1-5%, 2-8% and 72-117%, respectively. In total, 61 different organic and conventional beer samples from Germany and all over the world were analyzed by using the newly developed multi-mycotoxin method. In summary, deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyl deoxynivaleneol and enniatin B were quantified in rather low contents in the investigated beer samples. None of the other monitored Fusarium toxins like 15-acetyldeoxynivalenol, HT2- and T2-toxin, zearalenone, enniatin B1, A1, A or beauvericin were detectable.
外文关键词：Fusarium mycotoxins；Stable isotope dilution assay；LC-MS/MS；Solid phase extraction；Beer
作者：Habler, K；Gotthardt, M；Schuler, J；Rychlik, M
作者单位：Tech Univ Munich
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：218
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