中文摘要：氮氧化硅(SiON)微环谐振器可用于研发能检测牛奶中黄曲霉毒素M1 (AFM1)的生物传感器。针对硅烷表层与特异DNA-适配子或抗原结合片段Fab'相结合形成的两种不同的表面功能化策略，本研究进行了测试，并利用这两种策略制成具有亲和力的生物传感器。这2种功能化传感器均能对缓冲剂中的AFM1进行特异性检测，而不能对赭曲霉素进行非特异性检测。
 
外文摘要：Silicon oxynitride (SiON) microring resonators are used to develop a biosensor device to detect Aflatoxin M1 (AFM1), a carcinogenic mycotoxin present in milk. Two different surface functionalization strategies are tested to realize an affinity biosensing device. Both strategies are based on the formation of a silane surface layer able to bind either specific DNA-aptamers or antigen binding fragments (Fab'). The sensing device consists of an array of four SiON microring resonators, fiber-coupled to a vertical cavity surface emitting laser operating at 850 nm. Detection is realized with off-chip silicon photodetectors. With both functionalizations, we demonstrate a specific detection of AFM1 in buffer, while the nonspecific detection of Ochratoxin is not occurring. Repeatability and reproducibility of both functionalization strategies are discussed.
外文关键词：Aflatoxin M1；antibodies；aptamers；microring resonators
作者：Chalyan, T；Pasquardini, L；Gandolfi, D；Guider, R；Samusenko, A；Zanetti, M；Pucker, G；Pederzolli, C；Pavesi, L
作者单位：Univ Trento
期刊名称：IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
期刊影响因子：3.466
出版年份：2017
出版刊次：2
点击下载：用适配子或Fab'片段功能化的微环谐振器检测黄曲霉毒素M1