中文摘要：黄曲霉毒素B1（AFB1）是一种存在于食物和饲料中的最有害的霉菌毒素，容易致使人或动物患肝癌。因此，限制人和牲畜的黄曲霉毒素暴露非常有必要。本研究旨在从各种来源中分离黄曲霉毒素B1的解毒菌并进行鉴定，从而开发一种安全且环境友好的AFB1管理策略。研究发现，地衣芽孢杆菌CFR1的无细胞上清液比细胞裂解液更能有效地降解AFB1。本研究首次证实了地衣芽孢杆菌可以降解90%以上的AFB1。因此，地衣芽孢杆菌CFR1可以用于田间和食品基质中AFB1生物修复和脱毒。
外文摘要：Aflatoxin B1 is the most harmful among the mycotoxins commonly present in food and feed, and it may lead to hepatocellular carcinoma in humans and animals. Therefore, limiting its exposure to humans and livestock is very much essential. The present study aims to isolate and characterize Aflatoxin B1 detoxifying bacteria from various sources, to develop a safe and environment-friendly strategy for Aflatoxin B1 management. Fifty-six bacteria were isolated using a media amended with coumarin as a sole carbon source. Seven strains showed more than 70% reduction of AFB1 in liquid culture media. Among them, isolate CFR1 reduced Aflatoxin B1 by 94.7%, and it was selected for further studies. CFR1 was identified as Bacillus licheniformis CFR1, by biochemical characterization and 16S rRNA gene sequencing. The cell-free supernatant of B. licheniformis CFR1 was able to degrade AFB1 efficiently than the cell lysate. The degradation of AFB1 was examined using High-Performance Thin Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC) and Electron spray ionization-Mass Spectrometry (ESI-MS). The optimal temperature, time, and pH of the medium for the maximum degradation of Aflatoxin B1 were found to be 37 °C, 24 h and 7, respectively. Furthermore, Ames test for mutagenicity showed that when treated with B. licheniformis CFR1 extracellular fraction coincided with the loss of Aflatoxin B1 mutagenicity. To the best of our knowledge, this is the first study that shows more than 90% degradation of AFB1 by B. licheniformis. Thus, B. licheniformis CFR1 might be an excellent candidate for bioremediation and detoxification of Aflatoxin B1 from both field and food matrices.
外文关键词：Mycotoxin；Aflatoxin B-1；Degradation；Detoxification；Bacillus licheniformis
作者：Rao, K. Raksha; Vipin, A. V.; Hariprasad, P.; Appaiah, K. A. Anu
作者单位：CSIR Cent Food Technol Res Inst，India
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2017
出版刊次：1
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