中文摘要：花生在长期储存期间容易受到真菌感染，从而产生毒素危害人类健康。本研究旨在分离和鉴定花生中地真菌，并且利用银纳米粒子（AgNPs）抑制它们。研究中合成的所有AgNPs均具有抑制真菌生长的能力。香茅叶提取物介导的AgNPs对所有测试的真菌都呈现显著的抗真菌潜力，其最低抑菌浓度是20 μg/ml。本研究提出的生物方法具有生态友好、安全和经济可行性。AgNPs对花生产毒分离物具有显著得抗真菌活性，因此可以有效地用于产毒病原体的管理。
外文摘要：Peanuts are vulnerable to fungal infections during long term storage. Fungi infecting peanuts are toxigenic and cause health hazards. The goal of this study was isolation and identification of fungi in peanuts and use of silver nanoparticles (AgNPs) to inhibit them. For this purpose firstly, we have isolated fungi from infected peanuts and identified on the basis of morphological and molecular study. Out of the total 54 fungal isolates, 47 were found to be Aspergillus spp. and other belongs to Penicillium spp. and Macrophomina phaseolina. Biochemical assay was performed to identify cultures of Aspergillus flavus from other species of this genus by inoculating it on Aspergillus Differentiation Medium (ADM). Thirty-one isolates were found to be A. flavus. Toxicity of Aspergillus spp. was evaluated on Yeast Extract Sucrose agar (YES) medium with an additive methylated β-cyclodextrin and nine isolates were found toxigenic. Secondly, AgNPs were synthesized from ten different plants and their characterization was performed using various analytical techniques such as UV–Visible spectrophotometer, Fourier Transform Infrared Spectroscopy, Zetasizer and Nanoparticle Tracking Analysis, etc. Further, antifungal potential of thus synthesized AgNPs was evaluated. All the synthesized AgNPs possess ability to inhibit fungal growth. Cymbopogon citratus leaf extract mediated AgNPs were found to have prominent antifungal potential against all test fungi and its minimum inhibitory concentration (MIC) was found to be 20 μg/ml. The biogenic approach proposed in the present study is eco-friendly, safe and economical viable. AgNPs also reported to have significant antifungal activity against toxigenic isolates of peanuts, hence such AgNPs can be effectively used for the management of toxigenic pathogens.
外文关键词：Aspergillus differentiation medium；Antifungal；Methylated beta-cyclodextrins；MIC；Peanuts；Silver nanoparticles
作者：Jogee, Priti S.; Ingle, Avinash P.; Rai, Mahendra
作者单位：SGB Amravati Univ,India
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2017
出版刊次：1
点击下载：染病花生中分离和鉴定产毒真菌，以及银纳米粒子的杀菌性能