中文摘要：在转BT基因棉花中，Bt毒素的充分表达和种子纯度可控制棉铃虫，并因此提高棉花产量。本文通过对棉铃虫的生物鉴定和试纸条测定，来研究Bt毒素蛋白在不同转Bt基因棉花（JKCH 226, JKCH 1947, JKCH Durga, JKCH Ishwar, JKCH Varun KDCHH 441 和 KDCHH 621）F-1和F-2代的种子和叶片中的不同。研究发现，转BT基因棉花种子和叶片中的Bt素毒蛋白对棉铃虫的毒性一致。研究结果表明，为了转基因棉花的可持续生产，转基因棉花种子的性状纯度要达到95%。
外文摘要：Adequate expression of Bt (Bacillus thuringiensis) toxins and purity of seeds of Bt-transgenic cottons are important for controlling bollworms, and thereby increasing the cotton productivity. Therefore, we examined the variability in expression of Bt toxin proteins in the seeds and in leaves of different cotton (Gossypium hirsutum (L.) hybrids (JKCH 226, JKCH 1947, JKCH Durga, JKCH Ishwar, JKCH Varun KDCHH 441 and KDCHH 621) expressing Bt toxins in F-1 and F-2 generations, using bioassays against the cotton bollworm, Helicoverpa armigera (Hubner), and the lateral flow strip (LFS) test. Toxicity of Bt toxin proteins in the seeds of Bt-transgenic cottons to H. armigera correlated with their toxicity in the leaves in one toxin Bt cotton hybrids. The Bt-F-1 and Bt-F-2 seeds of JKCH 1947 were more toxic to H. armigera than those of JKCH Varun seeds. The seeds and leaves of F-1s showed greater toxicity than the F-2 seeds or leaves of one-toxin (crylAc) Bt cotton hybrids. However, no significant differences were observed for the two-toxin (crylAc and cry2Ab) hybrid, KDCHH 621. Toxicity of leaves to H. armigera increased with crop age, until 112 days after seedling emergence. The Bt trait purity in F-1 seeds of four two-toxin Bt cotton hybrids ranged from 86.7 to 100%. The present study emphasizes the necessity of 95% Bt trait purity in seeds of transgenic cotton for sustainable crop production.
外文关键词：Bacillus thuringiensis；Bt cotton hybrids；Cotton bollworm；Gossypium hirsutum; Helicoverpa armigera；Relative toxicity
作者：Singh, B. P.；Sandhu, S. S.；Kalia, V. K.；等
作者单位：ICAR Indian Agr Res Inst IARI
期刊名称：INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY
期刊影响因子：1.165
出版年份：2016
出版刊次：4
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