中文摘要：本研究利用LC-MS/MS法检测了207个小麦粉和玉米糁样品受白僵菌毒素和4种恩镰孢菌素（A、A1、B和B1）污染的情况。5种毒素的回收率范围为91.1%-113.8%。同时，用免疫亲和层析法检测了样品中的脱氧雪腐镰刀菌烯醇和雪腐镰刀菌烯醇，发现脱氧雪腐镰刀菌烯醇与恩镰孢菌素发生复合污染情况。研究结果表明在日本需要对环缩酚酸肽霉菌毒素进行风险评估并研究脱氧雪腐镰刀菌烯醇与恩镰孢菌素的协同效应。
 
外文摘要：The contamination levels of beauvericin and four enniatins, enniatin A, A1, B and B1, in a total of 207 samples of wheat flour and corn grits on the Japanese market were determined by an analytical method based on LC-MS/MS. The toxins were extracted from samples with acetonitrile-water (85:15, vol/vol) and then purified with C18 cartridges. The method was validated in a single laboratory using spiked samples at two levels, and the recovery of the five toxins ranged from 91.1 to 113.8%. Enniatin B was frequently detected in imported wheat flour (81.8%) and domestic wheat flour (85.6%), and the highest concentration of enniatin B was present in a domestic wheat sample (633 μg kg-1). In corn grits, beauvericin was found in 34% of the samples, but enniatins were not detected at all. The maximum concentration of beauvericin in corn grits was 26.1 μg kg-1. Deoxynivalenol and nivalenol in the same samples were determined by a method using an immunoaffinity column. Co-contamination of deoxynivalenol and enniatins was observed in 61% of the imported wheat samples and in 58% of the domestic wheat samples. These results suggest the need for a risk assessment for cyclic depsipeptide mycotoxins in Japan and a study about the synergistic effect of deoxynivalenol and enniatins.
外文关键词：Beauvericin；enniatin；deoxynivalenol；wheat；corn
作者：Yoshinari, T；Suzuki, Y；Sugita-Konishi, Y；Ohnishi, T；Terajima, J
作者单位：Natl Inst Hlth Sci
期刊名称：FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
期刊影响因子：1.878
出版年份：2016
出版刊次：10
点击下载：小麦粉和玉米糁受白僵菌毒素和恩镰孢菌素污染以及其与B类单端孢霉烯霉菌毒素复合污染的情况研究