中文摘要：黄曲霉毒素是最常见的、造成农作物污染的霉菌毒素，由黄曲霉、寄生曲霉等真菌产生。小麦是伊朗最重要的主食之一，而伊朗北部地区的环境条件非常适合真菌生长。本研究旨在检测伊朗北部戈勒斯坦省粮仓中小麦的黄曲霉毒素浓度。研究中，从戈勒斯坦省的3个粮仓中采集样品，用免疫亲和层析法分离出黄曲霉毒素后，再用高效液相色谱法和荧光检测器检测黄曲霉毒素的浓度。研究发现，34个样品中有10个检出了黄曲霉毒素污染，检出率为29.4%。小麦样品的湿度与是否受黄曲霉毒素污染无相关关系。研究结果表明，尽管样品中检测到的总黄曲霉毒素低于伊朗许可范围，但不容忽视的是，29%的黄曲霉毒素污染率仍然可能给健康带来负面影响。可以预见，随着储存时间的延长，黄曲霉毒素污染水平也将升高。
外文摘要：BACKGROUND: Aflatoxins are the most common mycotoxins that contaminate crops. They are produced by fungi such as Aspergillus flavus and Aspergillus parasiticus. Wheat (Tricitumaestivum) is one of the most important staple foods used in Iran, and the environmental conditions in the north of Iran are favorable to fungal growth. This study was designed in order to determine the aflatoxin concentration in wheat samples from silos in Golestan Province north of Iran. METHODS: Samples were collected from three silos of Golestan province. First, aflatoxins were isolated using immunoaffinity chromatography. Then the aflatoxin concentrations were determined by High performance liquid chromatography (HPLC) method and fluorescence detector. RESULTS: Ten out of 34 samples (29.4% of samples) were contaminated by aflatoxins.No concentration was found above permitted aflatoxin levels in Iran (15 ng/g). In one sample (2.9%), aflatoxin B1 was seen over the permissible limits in Iran. The highest level found in samples for total aflatoxin, aflatoxin B1, aflatoxin B2, aflatoxin G1 and aflatoxin G2 were 7.08 ng/g, 6.91 ng/g, 0.29 ng/g, 1.37 ng/g and 0.23 ng/g, respectively. No correlation was found between humidity levels in wheat samples contained aflatoxin and wheat samples without aflatoxin. CONCLUSION: Despite the total aflatoxins determined in samples were below the permissible limits in Iran, the 29% aflatoxin contamination rate can negatively affect health factors and it should not be neglected. So, it is predictable that if the storage duration of samples increases, the aflatoxin contamination levels will increase.
外文关键词：Aflatoxin；Wheat；HPLC；Iran
作者：Namjoo, M；Salamat, F；Rajabli, N；Haji-Hoseeini, R；Niknejad, F；Kohsar, F；Joshaghani, H
作者单位： Golestan Univ Med Sci
期刊名称：IRANIAN JOURNAL OF PUBLIC HEALTH
期刊影响因子：0.498
出版年份：2016
出版刊次：7
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