中文摘要：转基因生物的检测正在成为法律的必要，本文通过使用单一和多重PCR方法检测进口到伊朗的转基因大豆。作者首先从霍梅尼港市场获得了五种进口的大豆样品，然后用改良的CTAB法从大豆种子中提取DNA，并用PCR方法检测CaMV 35S启动子、NOS终止子、EPSPs基因的特异性引物。研究发现，从加拿大和巴拉圭进口的大豆样品是转基因作物，且其基因组里有CaMV 35S启动子、NOS终止子、EPSPs基因。单一PCR与多重PCR的检测结果相同，但多重PCR以更快、节省成本和时间的方式检测转基因大豆。基于转基因大豆特异性引物的PCR分析表明，该品种可能是GTS 40-3-2大豆。另外，在PCR检测中，当以美国或非转基因大豆的DNA为模板时，没有发现片段扩增。
外文摘要：The detection of Genetically Modified (GM) organisms is becoming a legal necessity. This study was carried out to detect genetically modified events in soybeans imported into Iran using simplex and multiplex PCR. Therefore, five samples of imported soybean were obtained from Bandar Imam Khomeini customs. Modified CTAB method was used to extract DNA from soybean seeds. The result indicates that the modified method is suitable for DNA extraction from soybean seeds and probably can be used for other oilseeds. Using specific primers for CaMV 35S promoter, NOS terminator and epsps gene PCR reactions were performed. In this study soybean lectin gene was used as internal control. The results revealed that soybean samples imported from Canada and Paraguay were genetically modified and they had CaMV 35S promoter, NOS terminator and epsps gene in their genomes. The result of simplex PCR was the same as multiplex PCR, but multiplex PCR detected the GM soybeans very quickly and in a cost-saving and time-consuming way. Based on PCR analysis using GM soybean event-specific primers, it is suggested that the soybean plants may be GTS 40-3-2. No fragment was amplified when the DNA of US or Non-GM soybeans were used as template in the PCR reaction. This is the first report that shows GM soybeans imported to Iran without use of the 'GMO' label in the shipment's documentation.
外文关键词：EPSPS gene；Genetically Modified Organisms (GMOs)；Monitoring of GMOs；Roundup ready soybean；Simplex and multiplex PCR
作者：Sarmadi, L；Alemzadeh, A；Ghareyazie, B
作者单位：Shiraz Univ
期刊名称：JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY
期刊影响因子：0.816
出版年份：2016
出版刊次：5