中文摘要：miRNA在真核生物基因调控中发挥根本作用。目前，还没有学者对转基因生物miRNA的组成进行研究。本文利用深度测序技术和生物信息学方法来研究两种转基因大豆及其亲本品种种子的miRNA。研究发现，转基因大豆和非转基因大豆的gma-miRNA表达有一些差异。另外，在远亲非转基因大豆间gma-miRNA表达的差异更大，这表明大豆种子miRNA的组成在不同的大豆品种间是不同的，差异的内容有可能是遗传的关系。本文的研究首次为食用转基因作物的miRNA组成提供了有用的数据，也为大豆miRNA的研究提供了有价值的信息。
外文摘要：MicroRNAs (miRNAs) have been widely demonstrated to play fundamental roles in gene regulation in most eukaryotes. To date, there has been no study describing the miRNA composition in genetically modified organisms (GMOs). In this study, small RNAs from dry seeds of two GM soybean lines and their parental cultivars were investigated using deep sequencing technology and bioinformatic approaches. As a result, several differentially expressed gma-miRNAs were found between the GM and non-GM soybeans. Meanwhile, more differentially expressed gma-miRNAs were identified between distantly related non-GM soybeans, indicating that the miRNA components of soybean seeds varied among different soybean lines, including the GM and non-GM soybeans, and the extent of difference might be related to their genetic relationship. Additionally, fourteen novel gma-miRNA candidates were predicted in soybean seeds including a potential bidirectionally transcribed miRNA family with two genomic loci (gma-miR-N1). Our findings firstly provided useful data for miRNA composition in edible GM crops and also provided valuable information for soybean miRNA research.
作者：Wang, Yong；Lan, Qingkuo；Zhao, Xin；等
作者单位：河北农业大学
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2016
出版刊次：5
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