中文摘要：食品、农产品和中药材等极易受到黄曲霉毒素（AFS）和赭曲霉毒素A（OTA）的侵害，目前已经造成了严重的世界性健康问题和经济问题。本文选择被真菌感染15天后的生姜作为实验样本，采用超声辅助固-液萃取技术、免疫亲和柱净化技术和UFLC-MS/MS法检测生姜中的AFs 和 OTA毒素。结果显示，AFs 和 OTA毒素的检测限为 0.04-0.30μg/mL，定量限为0.125-1.0μg/mL，回收率为82.0-100.2%。生姜样本感染15天后，并没有发现明显的霉变，但从生姜表皮（低于定量限）到最内层（51.86μg/mL）AFB(1) 含量在逐渐增加；只在生姜最内层皮中检测到AFB(2) 毒素，含量为0.87μg/mL。而且生姜感染后只在姜皮中检测到了非常少量（低于定量限）的OTA毒素。本文所构建方法成功检测出了生姜中的五种真菌毒素，而且具有快速、灵敏度高、安全等特点。
外文摘要：BACKGROUNDAflatoxins (AFs) and ochratoxin A (OTA) have been detected frequently in food, agricultural products and traditional Chinese medicines, and their presence poses serious health and economic problems worldwide. Ginger can easily be polluted with mycotoxins. In this study, ginger samples were cultivated for 15 days after inoculation with fungi and were prepared based on ultrasound-assisted solid-liquid extraction using methanol/water followed by immunoaffinity column clean-up and analysed by ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) for AFs and OTA. RESULTS The limits of detection and quantification of AFs and OTA were 0.04-0.30 mu g mL(-1) and 0.125-1.0 mu g mL(-1), respectively. The recoveries were 82.0-100.2%. After 15 days' cultivation, no macroscopic mildew was found in ginger. But, the content of AFB(1) expressed an increasing trend in ginger, peel [less than the limit of quantification (LOQ)] to the innermost layer (51.86 mu mL(-1) ), AFB(2) was only detected in the innermost layer at the level of 0.87 mu mL(-1). A small amount ( 外文关键词：UFLC-MS, MS, ginger, fungi inoculation, mycotoxins
作者：Yang, Ying; Wen, Jing; Kong, Weijun; Liu, Qiutao; Luo, Hongli; Wang, Jian; Yang, Meihua
作者单位： Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China.
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
期刊影响因子：2.076
出版年份：2016
出版刊次：9
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