中文摘要：本研究提出一种竞争性电位免疫方法用于食品中黄曲霉毒素B1（AFB1）的检测，并能提高检测结果的灵敏度。利用多克隆抗AFB1抗体 (pAb)对金纳米粒子(AuNPs; 16 nm i. d.)进行官能化，同时，通过在玻碳电极上固化AFB1-牛血清白蛋白复合物（AFB1-BSA）制备传感器电极。通过分析自然污染的花生样品验证了该方法的准确性，结果表明，本研究所提出的免疫测定法和商业酶联免疫吸附法的结果是一致的。
外文摘要：The authors report on a competitive potentiometric immunoassay for aflatoxin B1 (AFB1) in food that displays distinctly improved sensitivity. Gold nanoparticles (AuNPs; 16 nm i. d.) were functionalized with polyclonal anti-AFB1 antibody (pAb), whilst the sensor electrode was prepared by immobilizing AFB1-bovine serum albumin conjugate (AFB1-BSA) on a glassy carbon electrode. Upon addition of target AFB1, competitive immunobinding occurs between the analyte and AFB1-BSA for the labeled pAb on the AuNPs. The change in the surface charge as a result of the antigen-antibody reaction causes a shift in the electrical potential. With increasing concentrations of analyte (AFB1), the quantity of pAb-AuNP captured by the electrode decreases. The shift in the output potential is linearly proportional to the logarithm of AFB1 concentration in the 0.1 to 5.0 μg • kg−1 range, with a detection limit (LOD) of 87 ng • kg−1 (87 ppt). An intermediate precision of 10.9 % was accomplished in batch-to-batch identification. The selectivity over AFB2 with similar chemical structure is acceptable. The method accuracy was evaluated by analyzing naturally contaminated and spiked peanut samples, giving consistent results (with RSD values of <12 %) between this immunoassay and the commercial ELISA.
外文关键词：Electroanalysis;Biosensor;Bioconjugation;Immunosensor;Peanut analysis;Transmission electron microscopy;Batch-to-batch identification
作者：Li, Qunfang; Lv, Shuzhen; Lu, Minghua; Lin, Zhenzhen; Tang, Dianping
作者单位：河南大学
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2016
出版刊次：10
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