中文摘要：黄曲霉毒素B1（AFB1）是一种致癌物质，是世界范围内普遍存在的食品污染物。本研究利用高效液相色谱 - 电喷雾电离飞行时间质谱（HPLC/ESI/TOF/MS）方法，检测了有机酸将AFB1转化成AFB2a（一种未知的解毒物质）的能力。通过精确质量测定、与其它黄曲霉毒素的色谱分离、H1-核磁共振（NMR）和红外（IR）光谱等方法来证实转化产物的身份。研究发现，柠檬酸对AFB2a形成的影响最大。本研究结果为发现实用且有效的方法来降解受污染食物中黄曲霉毒素B1提供了基础。
外文摘要：Aflatoxin B1 (AFB1) is a class 1 carcinogen and a common food contaminant worldwide with widely uncontrolled human exposure. The ability of organic acids to transform AFB1 into a known detoxified form, aflatoxin B2a (AFB2a), was investigated using high performance liquid chromatography-electrospray ionisation-time of flight mass spectrometry (HPLC/ESI/TOF/MS). The identity of the transformation product was confirmed by accurate mass measurement, chromatographic separation from other aflatoxins, H1-nuclear magnetic resonance (NMR) and infrared (IR) spectroscopy. Of the weak acids tested, citric acid was found to be the most effective for AFB2a formation. At room temperature, 1 M citric acid was able to convert > 97% of AFB1 to AFB2a over 96 h of treatment. Up to 98% transformation was achieved by boiling AFB1 in the presence of citric acid for 20 min. AFB1 hydration after ingestion was explored by spiking AFB1 into simulated gastric fluid containing citric acid. Under these conditions, > 71% of AFB1 was hydrated to AFB2a and did not show any reversion to the parent compound after being transferred to a neutral solution. These results provide a basis for a practical and effective method for detoxification of AFB1 in contaminated foods.
外文关键词：High-performance liquid chromatography;time of flight mass s6pectrometry;aflatoxin;detoxification
作者：Rushing, Blake R.; Selim, Mustafa I.
作者单位：East Carolina Univ, USA
期刊名称：FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
期刊影响因子：1.878
出版年份：2016
出版刊次：9
点击下载：作为一种解黄曲霉毒素B1的方式，膳食酸对黄曲霉毒素B2a形成的影响