中文摘要：TAD1是在冬小麦低温驯化中产生并编码成具有抗菌活性的植物防御素。本文主要证实了重组TAD1蛋白可在体外抑制雪霉病菌和菌核病菌的菌丝生长。研究发现，过表达TAD1的转基因小麦可抵抗菌核病菌；叶片接种试验表明，过表达TAD1的转基因小麦可抵抗雪霉病，也可抵抗导致赤霉病的禾谷镰刀菌。研究结果表明，TAD1是禾谷类作物抵抗多种真菌病害的候选基因。
外文摘要：TAD1 (Triticum aestivum defensin 1) is induced during cold acclimation in winter wheat and encodes a plant defensin with antimicrobial activity. In this study, we demonstrated that recombinant TAD1 protein inhibits hyphal growth of the snow mold fungus, Typhula ishikariensis in vitro. Transgenic wheat plants overexpressing TAD1 were created and tested for resistance against T. ishikariensis. Leaf inoculation assays revealed that overexpression of TAD1 confers resistance against the snow mold. In addition, the TAD1-overexpressors showed resistance against Fusarium graminearum, which causes Fusarium head blight, a devastating disease in wheat and barley. These results indicate that TAD1 is a candidate gene to improve resistance against multiple fungal diseases in cereal crops. (C) 2016 Elsevier B.V. All rights reserved.
 
外文关键词：Cold acclimation；Defensin；Fusarium graminearum；Snow mold；Transgenic wheata
作者：Sasaki, Kentaro；Kuwabara, Chikako；Umeki, Natsuki；等
作者单位：Natl Agr & Food Res Org NARO
期刊名称：JOURNAL OF BIOTECHNOLOGY
期刊影响因子：2.667
出版年份：2016
出版刊次：6
点击下载：低温诱导防御素TAD1可使转基因小麦抵抗雪霉病和赤霉病