中文摘要：本文运用经过下一代测序分析的两个转座子系统（Sleeping Beauty 和Piggybac）及其基因组来高效繁殖转基因牛。11只转基因牛中有九只从产生到成长至今没有任何健康问题。另外，为了探讨转基因牛的基因变异，本文用NGS分析了全基因组的DNA。研究发现，在转基因牛的基因组中转座子组合被优先发现，且多只转基因牛中基因组的不稳定性没有显着性差异。研究结果表明，使用DNA转座子系统可以高效繁殖转基因牛，这些转基因牛可能是农业和兽医科学的宝贵资源。
外文摘要：Here, we efficiently generated transgenic cattle using two transposon systems (Sleeping Beauty and Piggybac) and their genomes were analyzed by next-generation sequencing (NGS). Blastocysts derived from microinjection of DNA transposons were selected and transferred into recipient cows. Nine transgenic cattle have been generated and grown-up to date without any health issues except two. Some of them expressed strong fluorescence and the transgene in the oocytes from a superovulating one were detected by PCR and sequencing. To investigate genomic variants by the transgene transposition, whole genomic DNA were analyzed by NGS. We found that preferred transposable integration (TA or TTAA) was identified in their genome. Even though multi-copies (i.e. fifteen) were confirmed, there was no significant difference in genome instabilities. In conclusion, we demonstrated that transgenic cattle using the DNA transposon system could be efficiently generated, and all those animals could be a valuable resource for agriculture and veterinary science.
 
作者：Yum, Soo-Young；Lee, Song-Jeon；Kim, Hyun-Min；等
作者单位：Seoul National University
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：5.228
出版年份：2016
出版刊次：6
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