中文摘要：为了给农民提供更好、更多的定制方案来提高产量，将多个转基因的性状整合到单一产品的做法越来越普遍。性状蛋白的表达水平可用于描述新型转基因产品和建立接触限值。荧光复用免疫测定方法（FMI）已在哺乳动物研究和临床应用中获得了广泛的认可。为了促进对堆积转基因性状的测量，本文开发了可以在堆积性状转基因品种的棉叶中检测五种蛋白的FMI方法，并对其进行了验证。研究发现，通过FMI方法测量得到的五种棉叶蛋白的表达水平与通过酶联免疫吸附法（ELISA）测量得到单个蛋白的表达水平是可评价和可比的；FMI方法与ELISA方法满足的需求特性类似。研究结果表明，在堆积性状转基因产品中，FMI方法与ELISA方法所得到蛋白表达水平是等同的，但FMI方法有更高的吞吐量、减少时间及更有效地利用资源的优势。
外文摘要：In order to provide farmers with better and more customized alternatives to improve yields, combining multiple genetically modified (GM) traits into a single product (called stacked trait crops) is becoming prevalent. Trait protein expression levels are used to characterize new GM products and establish exposure limits, two important components of safety assessment. Developing a multiplexed immunoassay capable of measuring all trait proteins in the same sample allows for higher sample throughput and savings in both time and expense. Fluorescent (bead-based) multiplexed immunoassays (FMI) have gained wide acceptance in mammalian research and in clinical applications. In order to facilitate the measurement of stacked GM traits, we have developed and validated an FMI assay that can measure five different proteins (beta-glucuronidase, neomycin phosphotransferase II, Cry1Ac, Cry2Ab2, and CP4 5-enolpyruvyl-shikimate-3-phosphate synthase) present in cotton leaf from a stacked trait product. Expression levels of the five proteins determined by FMI in cotton leaf tissues have been evaluated relative to expression levels determined by enzyme-linked immunosorbent assays (ELISAs) of the individual proteins and shown to be comparable. The FMI met characterization requirements similar to those used for ELISA. Therefore, it is reasonable to conclude that FMI results are equivalent to those determined by conventional individual ELISAs to measure GM protein expression levels in stacked trait products but with significantly higher throughput, reduced time, and more efficient use of resources.
 
外文关键词：fluorescent；multiplexed immunoassay；Luminex；ELISA；cotton；Gossypium hirsutum
作者：Yeaman, Grant R.; Paul, Sudakshina; Nahirna, Iryna; 等
作者单位：Monsanto Co
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
期刊影响因子：2.857
出版年份：2016
出版刊次：6
点击下载：用于检测棉花转基因蛋白质的荧光复用免疫测定方法的开发及验证