中文摘要：近年来随着市场上转基因生物数量的增加，迫切需要可用于分子检测技术的靶序列多样性。作为转基因生物分析的黄金标准，实时PCR技术可优化成为一种高通量转基因生物筛选方法。用这种方法，本文检测到19种转基因靶标。另外，通过来自20种转基因生物和4种非转基因生物的DNA特异性扩增发现，这种分析方法的特异性能达到100%。研究发现，这19种转基因靶标是在转基因生物中最常用的遗传要素，且在理论上能够在中国市场筛选已知的转基因生物。研究结果表明，该方法易用、快速、高效，且适合转基因检测实验室使用。
外文摘要：As the amount of commercially available genetically modified organisms (GMOs) grows recent years, the diversity of target sequences for molecular detection techniques are eagerly needed. Considered as the gold standard for GMO analysis, the real-time PCR technology was optimized to produce a high-throughput GMO screening method. With this method we can detect 19 transgenic targets. The specificity of the assays was demonstrated to be 100 % by the specific amplification of DNA derived from reference material from 20 genetically modified crops and 4 non modified crops. Furthermore, most assays showed a very sensitive detection, reaching the limit of ten copies. The 19 assays are the most frequently used genetic elements present in GM crops and theoretically enable the screening of the known GMO described in Chinese markets. Easy to use, fast and cost efficient, this method approach fits the purpose of GMO testing laboratories.
外文关键词：Genetically modified organism (GMO)；Identification; Screening；Real-time PCR
作者：Peng, Cheng；Wang, Pengfei；Xu, Xiaoli；等
作者单位：浙江农业科学院
期刊名称：SPRINGERPLUS
期刊影响因子：0.982
出版年份：2016
出版刊次：6
点击下载：一种可以检测19种靶标来识别转基因生物体的定性实时PCR方法研究