中文摘要：大豆花叶病毒（SMV）是最常见的大豆病毒，目前已经严重威胁到世界上大多数国家的大豆生产。本研究开发了一种非常实用且快速的大豆花叶病毒（SMV）胶体金试纸条分析（LFA）检测方法。本文研究结果显示，LFA检测方法可以用于检测受感染大豆叶片和种子中的花叶病毒，本研究所建立的方法具有实用、快速和特异性好等优点，能广泛应用于实验室或大田的SMV诊断和监视。
外文摘要：Soybean mosaic virus (SMV) is the most common virus in soybean and poses a serious threat to crop production and germplasm recession in many countries worldwide. In this study, a highly practical and rapid lateral-flow assay (LFA) was developed for the detection of SMV. The SMV coat protein (CP) was prokaryotically expressed and purified to immunize mice. After generation of hybridoma cell lines, four anti-SMV monoclonal antibodies were selected. The LFA-strip was then assembled using a double-antibody sandwich strategy. When the SMV-infected leaf sample was assayed using the assembled LFA-strip, the positive pink color appeared in the test line within 5-10 min. The strip only gave positive results with SMV and not other viruses tested and could be used to detect 800 fold dilutions of infected leaf samples. The LFA could be used to detect SMV in infected leaf tissue as well as soybean seeds. To our knowledge, this is the first report of the development of a LFA for the detection of SMV. The practical, rapid and specific assay that was developed in this study can be widely applied to the diagnosis and surveillance of SMV in the laboratory and the field. (C) 2016 Elsevier B.V. All rights reserved.
外文关键词：Soybean mosaic virus (SMV), Lateral-flow assay (LFA), Anti-SMV monoclonal antibody, Diagnosis
作者：Zhu, Min; Zhang, Wen-na; Tian, Jin-yan; Zhao, Wen-yang; Chen, Zheng-qiang; Sun, Li-hua; Xue, Fan; Liu, Yong; Tan, Xin-qiu; Wang, Li-min; Liu, Feng-quan; Tao, Xiao-rong
作者单位：Nanjing Agr Univ, Dept Plant Pathol, Minist Educ, Key Lab Integrated Management Crop Dis & Pests, Nanjing 210095, Jiangsu, Peoples R China.
期刊名称：JOURNAL OF VIROLOGICAL METHODS
期刊影响因子：1.508
出版年份：2016
出版刊次：9
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