中文摘要：本文用聚合酶链反应扩增和序列分析技术对中国地区254个来自农场和35个来自市场的大豆样品的外源基因进行了评估。研究发现，农场大豆样品中有67个CaMV35S阳性样本、46个NOS阳性样本、16个CP4-EPEPS阳性样本、66个BT阳性样本；市场大豆样品中有12个CaMV35S阳性样本、19个NOS阳性样本、13个CP4-EPEPS阳性样本、15个BT阳性样本。研究结果表明，在农场和市场的大豆样品中均检测到了外源基因，应谨慎管理转基因大豆的应用。
外文摘要："BACKGROUND: To evaluate the large quantities of genetically modified soybeans that are imported into China, we evaluated the presence of transgenes in soybean samples from an area, which are major soybean-producing areas.
We evaluated the presence of exogenous genes in a total of 254 soybean samples from farms and 35 commercially available soybean samples using polymerase chain reaction amplification and sequence analysis.
RESULTS: The farm soybean samples contained a total of 67 CaMV35S-positive samples, 46 NOS-positive samples, 16 CP4-EPEPS-positive samples, and 66 BT-positive samples. The commercially available soybean samples contained a total of 12 CaMV35S-positive samples, 19 NOS-positive samples, 13 CP4-EPEPS-positive samples, and 15 BT-positive samples.
CONCLUSION: Several exogenous genes were detected in the farmed and commercially available soybeans. We should use caution in the management of genetically modified soybeans. (C) 2015 Society of Chemical Industry"
外文关键词：genetically modified organisms；PCR detection；exogenous genes；soybean；sequence analysis
作者：Wang, Cunfang；Zhu, Yuying
作者单位：齐鲁工业大学
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
期刊影响因子：2.076
出版年份：2016
出版刊次：6
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