中文摘要：本研究开发了一种基于胶体金抗体探针的一站式竞争免疫层析检测法，可以快速检测水样中的吲哚美辛 （IDM）。通过试验鉴定，本方法测定吲哚美辛的时间少于10分钟，适用于现场快速测试。
外文摘要：One-step membrane-based competitive immunochromatographic assay using colloidal gold–antibody probe for rapid detection of indomethacin (IDM) in water samples was developed. A positive reaction as a result of the remaining gold–antibody conjugate combining with antigen coated on the membrane was obvious by visual detection. Under optimal conditions, the visual detection limit was found to be 0.1 ng mL−1, which was about 10–1000 times lower than those in published literatures, demonstrating the high sensitivity of the assay. The stability test indicated the immunochromatographic strips could be used for 8 weeks at room temperature without significant loss of activity. Real water samples were detected by the assay and confirmed by enzyme-linked immunosorbent assay. The assay time for indomethacin detection was less than 10 min, suitable for rapid testing on-site.
外文关键词：Fumonisin B-1;monoclonal antibody;immunochromatographic assay;detection;corn
作者：Wang, Xi-Chun; Fan, Hai-Xin; Fan, Meng-Xue; Li, Fu-Hui; Feng, Shi-Bin; Li, Jin-Chun; Wu, Jin-Jie; Li, Yu; Wang, Jia-Sheng
作者单位：安徽农业大学
期刊名称：FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
期刊影响因子：1.878
出版年份：2016
出版刊次：9
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