中文摘要：黄曲霉毒素是一种容易造成肝毒性、免疫抑制和抑制动物生长生产的真菌毒素。通过分析受污染的饲料基质进行黄曲霉毒素中毒病原诊断，这种方法具有显著的缺点，比如，饲料样品可能不具有代表性，而且基于饲料的测试方法不能进行病原诊断。基于组织的分析方法适用于接触生物标记，可以确认黄曲霉毒素。本研究利用荧光检测和柱前衍生化法开发并评价了一种实验室用的高效液相色谱法，可用于动物肝脏中的黄曲霉毒素M1, B1, B2, G1和G2。该方法用于检测肝脏中的黄曲霉毒素具有良好的选择性 ，且适用于肝脏中黄曲霉毒素M1，B1，B2，G1和G2的定量测定。
外文摘要：Aflatoxins are potent mycotoxins with effects that include hepatotoxicity, immunosuppression, and suppression of animal growth and production. The etiologic diagnosis of aflatoxicosis, which is largely based on analysis of contaminated feed matrices, has significant disadvantages given the fact that representative feed samples may not be available and feed-based test methods are not confirmatory of an etiologic diagnosis. A tissue-based analytical method for biomarkers of exposure would be valuable for confirmation of aflatoxicosis. We describe in-house development and evaluation of a high-performance liquid chromatographic method with fluorescence detection and precolumn derivatization for determination of aflatoxins M1, B1, B2, G1, and G2 in animal liver. The method demonstrates good selectivity for the tested aflatoxins in the liver matrix. The overall range was 0.03-0.10 ng/g for limit of detection and 0.09-0.18 ng/g for limit of quantitation. The correlation coefficient (R2) of calibration curves was >0.9978 for AFM1, 0.9995 for AFB1, 0.9986 for AFB2, 0.9983 for AFG1, and 0.9980 for AFG2 For fortification levels of 0.2-10 ng/g, repeatability was 10-18% for AFM1, 7-14% for AFB1, 5-14% for AFB2, 6-16% for AFG1, and 10-15% for AFG2 Recovery was 52-57% for AFM1, 54-62% for AFB1, 55-61% for AFB2, 57-67% for AFG1, and 61-65% for AFG2 There was no liver matrix effect found. The method is rugged against minor changes based on the selected factors. The results indicate that the proposed method is suitable for quantitative determination of aflatoxins M1, B1, B2, G1, and G2 in liver.
外文关键词：Aflatoxin;chromatography;evaluation;fluorescence;liver
作者：Shao, Dahai; Imerman, Paula M.; Schrunk, Dwayne E.; Ensley, Steve M.; Rumbeiha, Wilson K.
作者单位：Iowa State Univ,USA
期刊名称：JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
期刊影响因子：1.196
出版年份：2016
出版刊次：11
点击下载：一种实验室用高效液相色谱 - 荧光法的开发与评价，该方法可用于检测和定量分析动物肝脏中黄曲霉毒素M1, B1, B2, G(1)和G(2) 。