中文摘要：层出镰刀菌（F. proliferatum）作为丝状真菌病原体可以产生霉菌毒素，可以污染采后的果实，从而影响人类健康。本研究比较分析了在不存在和存在丁基羟基苯甲醚 (BHA) 的情况下，F. proliferatum的细胞外蛋白质组。研究结果表明，BHA处理可以通过抑制毒性因子和细胞壁降解酶的水平影响F. proliferatum的致病能力。另外，在BHA处理后，诱导与生长有关的蛋白质结果表明，F. proliferatum的生存可能与致病能力降低有关。本研究为理解F. proliferatum感染的复杂机制提供了证据，以寻找新的方法来控制这类真菌病原体。
外文摘要：Fusarium proliferatum as a filamentous fungal pathogen can produce mycotoxins that can contaminate postharvest fruits and thus impact risks on human health. The extracellular proteomes of F. proliferatum grown in the absence and presence of butylated hydroxyanisole (BHA) were analyzed comparatively. A total of 66 significantly different expressed secreted proteins were identified by LC-ESI-MS/MS analysis. The BHA treatment suppressed the accumulation of some pathogenic factors such as aspartic protease, cell wall degradation enzymes, porin, superoxide dismutase and glyceraldehyde-3-phosphate dehydrogenase. On the contrary, the BHA treatment increased the abundances of some proteins, such as ATP binding cassette transporter substrate-binding protein and lipopolysaccharide-assembly lipoprotein, involved in the growth of F. proliferatum. These findings suggest that BHA treatment could influence the pathogenic ability of F. proliferatum via inhibiting the levels of virulence factors and cell wall degradation-associated enzymes. Moreover, the induction of the growth-related proteins after the BHA treatment suggests that the livelihood of F. proliferatum might depend on the cost of reduced pathogenic ability. This study has provided some evidence for understanding the complicated mechanisms of F. proliferatum infection in an effort to develop new targets for the control of this fungal pathogen.
外文关键词：Fusarium proliferatum;Butylated hydroxyanisole;Proteomic;Secretome;Pathogenic ability;Antifungal agent Chinese Acad Sci,
作者：Li, Taotao; Jian, Quie; Wang, Yong; Chen, Feng; Yang, Chengwei; Gong, Liang; Duan, Xuewu; Yang, Bao; Jiang, Yueming
作者单位：中国科学院
期刊名称：JOURNAL OF PROTEOMICS
期刊影响因子：3.867
出版年份：2016
出版刊次：10
点击下载：基于比较蛋白质组学的丁羧基苯甲醚（BHA）对层出镰刀菌感染的抑制机制研究