中文摘要：由黄曲霉产生的玉米黄曲霉毒素污染对动物和人类健康而言是一种严重的危害。为了定位与抗黄曲霉相关的QTLs，作者采用了一种不同于以往的方法，该方法综合了全基因组关联分析 (GWAS)与传统连锁图谱分析两种方法的优点。研究中，利用228个重组自交系（RIL）进行图谱分析，并且定位了一个影响黄曲霉毒素累积的重要QTL（qAA8）。 qAA8的高分辨率图谱将有助于黄曲霉抗性的标记辅助选择（MAS）及致病基因的表征。
外文摘要：Maize grain contamination with aflatoxin from Aspergillus flavus (A. flavus) is a serious health hazard to animals and humans. To map the quantitative trait loci (QTLs) associated with resistance to A. flavus, we employed a powerful approach that differs from previous methods in one important way: it combines the advantages of the genome-wide association analysis (GWAS) and traditional linkage mapping analysis. Linkage mapping was performed using 228 recombinant inbred lines (RILs), and a highly significant QTL that affected aflatoxin accumulation, qAA8, was mapped. This QTL spanned approximately 7 centi-Morgan (cM) on chromosome 8. The confidence interval was too large for positional cloning of the causal gene. To refine this QTL, GWAS was performed with 558,629 single nucleotide polymorphisms (SNPs) in an association population comprising 437 maize inbred lines. Twenty-five significantly associated SNPs were identified, most of which co-localised with qAA8 and explained 6.7% to 26.8% of the phenotypic variation observed. Based on the rapid linkage disequilibrium (LD) and the high density of SNPs in the association population, qAA8 was further localised to a smaller genomic region of approximately 1500 bp. A high-resolution map of the qAA8 region will be useful towards a marker-assisted selection (MAS) of A. flavus resistance and a characterisation of the causal gene.
外文关键词：Aspergillus;flavus (A.flavus);genome-wide association analysis (GWAS);linkage mapping;maize;molecular marker;quantitative trait locus (QTL);recombinant inbred line (RIL)
作者：Zhang, Yu; Cui, Min; Zhang, Jimin; Zhang, Lei; Li, Chenliu; Kan, Xin; Sun, Qian; Deng, Dexiang; Yin, Zhitong
作者单位：扬州大学
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：9
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