中文摘要：脱氧雪腐镰刀菌烯醇(DON)由植物病原镰刀菌和大刀镰刀菌产生，是一种污染谷物和谷物制品最常见的霉菌毒素。食品和饲料的全球污染给人类和动物健康造成威胁，猪特别容易受到这种霉菌毒素的危害。DON衍生物（比如DOM-1）是由某些肠道细菌转化而来的，这些肠道细菌可能是自然发生的，也可能是食品添加剂造成的。肠上皮细胞是这些毒素的天然屏障。已有研究证实了DON对MAPK p44/42的诱导激活作用以及MAPK抑制剂（U0126 monoethanolate）对p44/42的抑制作用。本研究评估了DON和DOM-1对跨膜电阻(TEER)的影响，紧密连接蛋白7的活力和表达，以及作为抑制DON诱导效应的U0126。DON对TEER和claudin-3的影响至少能够部分介导p44/42 ，而对存活率和claudin-1的影响可能通过代替途径介导。
外文摘要：Deoxynivalenol (DON), produced by the plant pathogens Fusarium graminearum and Fusarium culmorum, is one of the most common mycotoxins, contaminating cereal and cereal-derived products. Although worldwide contamination of food and feed poses health threats to humans and animals, pigs are particularly susceptible to this mycotoxin. DON derivatives, such as deepoxy-deoxynivalenol (DOM-1), are produced by bacterial transformation of certain intestinal bacteria, which are naturally occurring or applied as feed additives. Intestinal epithelial cells are the initial barrier against these food- and feed-borne toxins. The present study confirms DON-induced activation of MAPK p44/42 and inhibition of p44/42 by MAPK-inhibitor U0126 monoethanolate. Influence of DON and DOM-1 on transepithelial electrical resistance (TEER), viability and expression of seven tight junction proteins (TJ), as well as the potential of U0126 to counteract DON-induced effects, was assessed. While DOM-1 showed no effect, DON significantly reduced TEER of differentiated IPEC-J2 and decreased expression of claudin-1 and -3, while leaving claudin-4; ZO-1, -2, and -3 and occludin unaffected. Inhibition of p44/42 counteracted DON-induced TEER decrease and restored claudin-3, but not claudin-1 expression. Therefore, effects of DON on TEER and claudin-3 are at least partially p44/42 mediated, while effects on viability and claudin-1 are likely mediated via alternative pathways.
外文关键词：transepithelial electrical resistance;mycotoxin;IPEC-J2;tight junctions;deoxynivalenol;de-epoxy deoxynivalenol
作者：Springler, Alexandra; Hessenberger, Sabine; Schatzmayr, Gerd; Mayer, Elisabeth
作者单位：Biomin Res Ctr, Austria.
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：9
点击下载：早期活化的MAPK p44/42 部分参与了脱氧雪腐镰刀菌烯醇（DON）引起的肠屏障功能和紧密连接网络破坏作用。