中文摘要：Laccases (LCs)是一种多铜氧化酶，可作为多功能生物催化剂对环境污染和外源性物质进行绿色生物修复。本研究阐明了来自秀珍菇的Lac2纯酶对黄曲霉毒素B1和M1的降解活性。研究证实了Lac2活性与黄曲霉毒素降解之间的关系。本研究的新颖性在于：首次鉴定出一种可以降解AFB1和AFM1的纯酶，并为通过天然酚类化合物介导进行有效降解的机制提供了证据。本研究结果为Lac2作为AFB1和AFM1的生物转化剂在食品和饲料供应链中的应用提供了新的视角。
外文摘要：Laccases (LCs) are multicopper oxidases that find application as versatile biocatalysts for the green bioremediation of environmental pollutants and xenobiotics. In this study we elucidate the degrading activity of Lac2 pure enzyme form Pleurotus pulmonarius towards aflatoxin B1 (AFB1) and M1 (AFM1). LC enzyme was purified using three chromatographic steps and identified as Lac2 through zymogram and LC-MS/MS. The degradation assays were performed in vitro at 25 °C for 72 h in buffer solution. AFB1 degradation by Lac2 direct oxidation was 23%. Toxin degradation was also investigated in the presence of three redox mediators, (2,2′-azino-bis-[3-ethylbenzothiazoline-6-sulfonic acid]) (ABTS) and two naturally-occurring phenols, acetosyringone (AS) and syringaldehyde (SA). The direct effect of the enzyme and the mediated action of Lac2 with redox mediators univocally proved the correlation between Lac2 activity and aflatoxins degradation. The degradation of AFB1 was enhanced by the addition of all mediators at 10 mM, with AS being the most effective (90% of degradation). AFM1 was completely degraded by Lac2 with all mediators at 10 mM. The novelty of this study relies on the identification of a pure enzyme as capable of degrading AFB1 and, for the first time, AFM1, and on the evidence that the mechanism of an effective degradation occurs via the mediation of natural phenolic compounds. These results opened new perspective for Lac2 application in the food and feed supply chains as a biotransforming agent of AFB1 and AFM1.
外文关键词：laccase;Pleurotus;mycotoxins;aflatoxin B-1;aflatoxin M-1;biodegradation;redox mediators
作者：Loi, Martina; Fanelli, Francesca; Zucca, Paolo; Liuzzi, Vania C.; Quintieri, Laura; Cimmarusti, Maria T.; Monaci, Linda; Haidukowski, Miriam; Logrieco, Antonio F.; Sanjust, Enrico; Mule, Giuseppina
作者单位：Natl Res Council Italy CNR,Italy.
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：9
点击下载：来自秀珍菇和氧化还原介质的Lac2对黄曲霉毒素B1和M1的降解作用研究