中文摘要：本文的研究的目的是评估RNA介导抗性的转基因小麦N-12对根际微生物群落的影响。本文将整个生长季节的冬小麦在两个地区进行了田间试验，且收集四个发育阶段（即幼苗期、拔节期、开花期和成熟期）的根际土壤，并利用16S rRNA基因的变性梯度凝胶电泳技术（DGGE）、DNA序列分析方法和BIOLOG Ecoplates（TM）分析方法研究了不同样品的根际微生物群落。DGGE图谱显示，在不同的生长季节根际微生物的群落结构有显著差异，而不同基因型作物的根际微生物群落结构无差异。另外，典范对应分析（CCA）结果表明，根际细菌多样性与各种环境因素高度相关，包括水分含量、速效磷含量和pH值。研究结果表明，不同因子（如生长期、土壤理化性质和田间类型）对小麦根际微生物群落的影响显著，而转基因小麦与其亲本株系之间无显著性差异。
外文摘要：The aim of this study was to evaluate the effects of RNA-mediated resistance in the transgenic wheat N-12 on rhizosphere microbial communities. Field experiments were performed in two different fields during the entire growing season of winter wheat, and the fields were in a principal wheat yellow mosaic disease area in the eastern region of China. Rhizosphere soils were collected at four developmental stages (i.e., seedling stage, stem elongation stage, flowering stage, and ripening stage), and the rhizosphere microbial communities of various samples were investigated using denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes followed by DNA sequence analysis and Biolog-EcoPlates (TM) analysis. The General Linear Model (GLM) combined with unweighted pair group method using average linkages (UPGMA) based on the DGGE profiles revealed significant differences based on growing seasons, while no differences in rhizosphere microbial community structures were detected between the crop genotypes. In addition, Canonical correspondence analysis (CCA) results indicated that the rhizosphere bacterial diversity was highly correlated with various environmental factors, including water content, available phosphorus and pH. Principal component analysis (PCA) of the Biolog data demonstrated that seasonal shifts in rhizosphere microbial communities were observed in both treatments. In conclusion, wheat rhizosphere microbial communities were significantly influenced by different factors, such as growing stage, soil physical and chemical properties and field type, and no differences were observed between the transgenic wheat and its parental line.
外文关键词：Wheat；Wheat yellow mosaic virus；DGGE；Biolog-EcoPlates (TM)；Rhizosphere microbial communities；RNA-mediated resistance
作者：Zhang, Zhenhua；Liu, Yan；Yu, Cigang；等
作者单位：南京环境科学研究所
期刊名称：APPLIED SOIL ECOLOGY
期刊影响因子：2.67
出版年份：2016
出版刊次：9
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