中文摘要：转基因成分在全世界各种食品中的使用逐渐增加。本文用定量实时PCR方法检测了五种转基因大豆品种（RRS，MON89788，a2704-12，a5547-127，MON87701）以监测其在香肠制品中的使用。作者从韩国的食品市场上收集了30种标签含有大豆成分的香肠制品，并对其进行了分析。实时PCR表明，在30种香肠制品中阈值周期值范围为28.62-33.90；有19种香肠制品不含有转基因大豆，在11种香肠制品中检测到了三种转基因大豆品种（RRS，MON89788，a2704-12）。研究结果表明，香肠制品中含有不同的转基因大豆品种。
外文摘要：The use of genetically modified (GM) ingredients in various types of food products has increased worldwide. In this study, qualitative real-time PCR assays targeting five GM soybean events (RRS, MON89788, A2704-12, A5547-127, and MON87701) were performed to monitor their use in sausage samples. Thirty sausage samples containing soybean ingredients on their label were collected from Korean food markets and analyzed. The endogenous lectin gene of soybean was used as an internal positive control. Real-time PCR showed that the threshold cycle (Ct) values ranged from 28.62 to 33.90 for the 30 sausage samples. Of the 30 sausage samples, 19 samples were negative for the presence of GM soybeans. In the positive samples, the results revealed three GM soybean events [Roundup Ready Soybean (RRS), A2704-12, and/or MON89788] in 11 of the 30 food samples tested. These results demonstrate that sausage products contain different GM soybean events.
外文关键词：Genetically modified soybean；Real-time PCR；Monitoring; Sausage
作者：Kim, Jae-Hwan；Song, Ji-Yeon；Hong, Yewon；等
作者单位：KYUNG HEE UNIVERSITY
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2016
出版刊次：9
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