中文摘要：本文通过全基因组序列分析方法对未知转基因木瓜的转基因序列进行了识别。作者用实时聚合酶链反应（PCR）对新鲜的转基因木瓜进行检测从而生成全基因组序列数据，并将所获得的序列数据映射到一个开放的数据库，从而获得木瓜基因组序列。转基因木瓜的转基因构件序列和品系特异性序列可抵抗番木瓜环斑病毒的感染。本文基于转基因序列开发了一种适用于各种食品的转基因木瓜特异性实时PCR检测方法。全基因组序列分析可在转基因木瓜中识别未知的转基因构件序列和品系特异性序列，且是在木瓜食品中检测转基因构件序列和品系特异性序列的可靠方法。
外文摘要：Identification of transgenic sequences in an unknown genetically modified (GM) papaya (Carica papaya L.) by whole genome sequence analysis was demonstrated. Whole genome sequence data were generated for a GM-positive fresh papaya fruit commodity detected in monitoring using real-time polymerase chain reaction (PCR). The sequences obtained were mapped against an open database for papaya genome sequence. Transgenic construct-and event-specific sequences were identified as a GM papaya developed to resist infection from a Papaya ringspot virus. Based on the transgenic sequences, a specific real-time PCR detection method for GM papaya applicable to various food commodities was developed. Whole genome sequence analysis enabled identifying unknown transgenic construct-and event-specific sequences in GM papaya and development of a reliable method for detecting them in papaya food commodities.
外文关键词：Genetically modified；Transgenic sequence；Next-generation sequencer；Genome sequence；Real-time PCR；Carica papaya L
作者：Nakamura, Kosuke； Kondo, Kazunari；Akiyama, Hiroshi；等
作者单位：Natl Inst Hlth Sci
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2016
出版刊次：8
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