中文摘要：本文研究了黄曲霉毒素B1和总伏马菌素及其产毒真菌对埃塞俄比亚东哈勒尔盖地区新收及储存高粱籽的自然污染情况。 用酶联免疫吸附法(ELISA)测定霉菌毒素含量，检测限（LOD）为0.01-0.03gkg(-1)。结果发现所有样品均同时受到曲霉菌和镰刀菌污染。黄曲霉毒素B1含量范围在低于LOD到33.10 mu gkg(-1)之间。储存高粱籽中黄曲霉毒素B1含量明显高于新收样品。所有样品中总伏马菌素含量在907-2041 mu gkg(-1)之间，其中最低含量在新收样品中。高粱是受研究区人们的主食谷物，每人每天的摄入量很大，长期采食低污染的粮食，摄入的霉菌毒素可能导致慢性中毒。
外文摘要：Natural contamination of sorghum grains by aflatoxin B1 and total fumonisin and their producing toxigenic fungi has been studied. A total of 90 sorghum grain samples were collected from small scale farmers' threshing floors and 5 to 6 months later from underground pits during 2013 harvest from 3 districts of East Hararghe, Ethiopia. Mycotoxin analysis was done using enzyme linked immunosorbent assay (ELISA). The limits of detection were in the range 0.01-0.03 μg kg-1. The results revealed that all sorghum grain samples were contaminated with both Aspergillus and Fusarium species. Aflatoxin B1 was detected at levels ranging from -1 grain. There were marked variations in aflatoxin B1 concentrations between fresh and stored samples, with much higher levels in the latter. Total fumonisin levels varied between 907 and 2041 µg kg-1 grain across the samples. Lowest total fumonisin was recorded in freshly harvested sorghum grain samples. Sorghum is a main staple cereal in the studied districts and its consumption per day per person is high. Daily intake of low doses of mycotoxin contaminated food stuff over a period of time could lead to chronic mycotoxicosis.
外文关键词：aflatoxin；ELISA；fumonisins；mycotoxicosis； sorghum
作者：Taye, W；Ayalew, A；Chala, A；Dejene, M
作者单位：Wolaita Sodo Univ
期刊名称：FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE
期刊影响因子：1.467
出版年份：2016
出版刊次：4
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