中文摘要：本研究优化开发了一种荧光偏振免疫测定法，并将其用于快速检测燕麦、大麦和黑麦几种谷物，及人类直接消费的燕麦片、燕麦薄饼和意面等谷物制品中的T-2和HT-2毒素。结果显示，优化后的测定方法，结合了快速与简易的特性，适用于高通量筛选及定量测定谷物及谷物制品中的T-2和HT-2毒素。
外文摘要：A fluorescence polarization (FP) immunoassay has been optimized and validated for rapid quantification of T-2 and HT-2 toxins in both unprocessed cereals, including oats, barley and rye, and cereal-based products for direct human consumption, such as oat flakes, oats crispbread and pasta. Samples were extracted with 90 % methanol, and the extract was filtered and diluted with water or sodium chloride solution prior to the FP immunoassay. Overall mean recoveries from spiked oats, rye, barley, oat flakes, oats crispbread and pasta ranged from 101 to 107 %, with relative standard deviations lower than 7 %. Limits of detection (LODs) of the FP immunoassay were 70 μg/kg for oats, 40 μg/kg for oat flakes and barley, 25 μg/kg for pasta and 20 μg/kg for rye and oats crispbread. The trueness of the immunoassay was assessed by using two oat and oat flake reference materials for T-2 and HT-2 toxins, showing good accuracy and precision. Good correlations (r > 0.953) were observed between T-2 and HT-2 toxin contents in naturally and artificially contaminated samples determined by both FP immunoassay and ultra-high-performance liquid chromatography (UHPLC) with immunoaffinity column cleanup used as reference method. These results, combined with rapidity and simplicity of the assay, show that the optimized assay is suitable for high-throughput screening, as well as for reliable quantitative determination of T-2 and HT-2 toxins in cereals and cereal-based products.
Keywords:T-2 and HT-2 toxins, Fluorescence polarization immunoassay, Oats, Barley, Rye, Cereal-based products
外文关键词：T-2 and HT-2 toxins；Fluorescence polarization immunoassay；Oats；Barley；Rye；Cereal-based products
作者：Porricelli, ACR；Lippolis, V；Valenzano, S；Cortese, M；Suman, M；Zanardi, S；Pascale, M
作者单位：Natl Res Council Italy CNR
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2016
出版刊次：12
点击下载：一种可用于谷物及谷物制品中T-2和HT-2毒素快速检测的荧光偏振免疫测定法的优化与验证
