中文摘要：本文利用便携式纳米结构成像表面等离子体共振（iSPR）生物传感器（又称光子成像），研究了一种可用于分析啤酒中霉菌毒素脱氧雪腐镰刀菌烯醇（DON）和赭曲霉毒素A（OTA）的竞争抑制法。啤酒中DON和OTA的检测限分别为17 ng/mL和7 ng/mL，能检测出低于每日耐受量的１０％的DON和OTA。对２０个不同批次的啤酒进行初步的内部验证，显示该传感器能检测啤酒中的这两种毒素。该方法检测啤酒中DON时不需要预浓缩，可用于现场或在线检测；而检测OTA时，则需浓缩，因而不适于现场检测。
外文摘要：A competitive inhibition immunoassay is described for the mycotoxins deoxynivalenol (DON) and ochratoxin A (OTA) in beer using a portable nanostructured imaging surface plasmon resonance (iSPR) biosensor, also referred to as imaging nanoplasmonics. The toxins were directly and covalently immobilized on a 3-dimensional carboxymethylated dextran (CMD) layer on a nanostructured iSPR chip. The assay is based on competition between the immobilized mycotoxins and free mycotoxins in the solution for binding to specific antibodies. The chip surface was regenerated after each cycle and the combination of CMD and direct immobilization of toxins allowed the chips to be used for more than 450 cycles. The limits of detection (LODs) in beer were 17 ng/mL for DON and 7 ng/mL for OTA (or 0.09 ng/mL after 75 times enrichment). These LODs allowed detection of even less than 10% exhaustion of the tolerable daily intake of DON and OTA by beer. Significant cross-reactivity of anti-DON was observed towards DON-3-glucoside and 3-acetyl-DON while no cross-reactivity was seen for 15-acetyl-DON. A preliminary in-house validation with 20 different batches of beer showed that both toxins can be detected at the considered theoretical safe level for beer. The assay can be used for in-field or at-line detection of DON in beer and also in barley without pre-concentration, while OTA in beer requires an additional enrichment step thus making the latter in its present form less suitable for field applications.
外文关键词：imaging SPR；mycotoxins；beer；nanoplasmonics；deoxynivalenol；ochratoxin A
作者：Joshi, S；Annida, RM；Zuilhof, H；van Beek, TA；Nielen, MWF
作者单位：Wageningen Univ
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
期刊影响因子：2.857
出版年份：2016
出版刊次：43
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