中文摘要：CpBV-CST1病毒基因是从菜蛾盘绒茧蜂多分DNA病毒（CpBV）中检测到的，其蛋白产物对鳞翅目昆虫具有明显的毒性。本文研发了一种表达CpBV-CST1基因的转基因烟草。研究发现，转基因烟草T1代的mRNA和蛋白质中存在CpBV-CST1基因；甜菜夜蛾幼虫取食转基因烟草后的死亡率增高。为进一步探讨转基因烟草的抗虫性，作者用生物测定对转基因烟草的排趋性和抗生性进行了评估。在选择试验中，甜菜夜蛾幼虫明显避免在T1代植物上取食。饲喂T1代植物的甜菜夜蛾幼虫的蛋白酶活性明显降低，这可能是因为其摄取了转基因植物的CpBV-CST1蛋白。此外，转基因烟草对其他危害烟草的昆虫也表现出了相似的抗虫性。研究结果表明，CpBV-CST1病毒基因可用于转基因抗虫植物的研发中，建议扩大该转基因方法来获得高价值的作物。
外文摘要：A viral gene, CpBV-CST1, was identified from a polydnavirus Cotesia plutellae bracovirus (CpBV). Its protein product was significantly toxic to lepidopteran insects. This study generated a transgenic tobacco plant expressing CpBV-CST1. Expression of transgene CpBV-CST1 was confirmed in T1 generation (second generation after transgenesis) in both mRNA and protein levels. Young larvae of Spodoptera exigua (Hubner) suffered high mortalities after feeding on transgenic tobacco. All 10 T1 transgenic tobacco plants had no significant variation in speed-to-kill. In order to further explore insect resistance of these transgenic tobaccos, bioassays were performed by assessing antixenosis and antibiosis. S. exigua larvae significantly avoided T1 plants in a choice test. Larvae fed with T1 plant exhibited significant decrease in protease activity in the midgut due to consuming CpBV-CST1 protein produced by the transgenic plant. Furthermore, the transgenic tobacco exhibited similar insect resistance to other tobacco-infesting insects, including a leaf-feeding insect, Helicoverpa assulta, and a sap-feeding insect, Myzus persicae. These results demonstrate that a viral cystatin gene can be used to develop insect-resistant transgenic plant, suggesting a prospective possibility of expanding the current transgenic approach to high-valued crops.
外文关键词：transgenic tobacco；polydnavirus；antibiosis；Spodoptera exigua；cystatin
作者：Kim, E；Kim, Y；Yeam, I；Kim, Y
作者单位：Andong Natl Univ
期刊名称：ENVIRONMENTAL ENTOMOLOGY
期刊影响因子：1.315
出版年份：2016
出版刊次：10
点击下载：在转基因烟草中半胱氨酸蛋白酶抑制剂病毒基因（CpBV-CST1）的表达可提高烟草的抗虫性