中文摘要：本文通过（1）研究喂养90天转基因水稻的SD大鼠的基因水平转移（HGT）；（2）检测Cry1C蛋白对体外消化性和致敏性的影响；和（3）用急性和亚慢性毒性试验研究喂养转基因水稻T1C-1的大鼠肠道菌群的变化，对转Cry1C基因水稻T1C-1进行安全评价。研究发现，在转Bt基因水稻T1C-1检测中没有发现鼠和转基因水稻间的基因水平转移；Cry1C蛋白的序列搜索没有发现已知过敏原或毒素的同源序列；Cry1C蛋白在体外模拟胃液和肠液试验中迅速降解。在大鼠中表达Cry1C蛋白没有产生高特异性的IgG和IgE抗体。另外，作者通过DGGE分析来进行大鼠肠道菌群的急性和亚慢性毒性试验。DGGE图谱的聚类分析显示，大鼠的肠道菌群有差异个体显著。
外文摘要：In this study, assessment of the safety of transgenic rice T1C-1 expressing Cry1C was carried out by: (1) studying horizontal gene transfer (HGT) in Sprague Dawley rats fed transgenic rice for 90 d; (2) examining the effect of Cry1C protein in vitro on digestibility and allergenicity; and (3) studying the changes of intestinal microbiota in rats fed with transgenic rice T1C-1 in acute and subchronic toxicity tests. Sprague Dawley rats were fed a diet containing either 60% GM Bacillus thuringiensis (Bt) rice T1C-1 expressing Cry1C protein, the parental rice Minghui 63, or a basic diet for 90 d. The GM Bt rice T1C-1 showed no evidence of HGT between rats and transgenic rice. Sequence searching of the Cry1C protein showed no homology with known allergens or toxins. Cry1C protein was rapidly degraded in vitro with simulated gastric and intestinal fluids. The expressed Cry1C protein did not induce high levels of specific IgG and IgE antibodies in rats. The intestinal microbiota of rats fed T1C-1 was also analyzed in acute and subchronic toxicity tests by DGGE. Cluster analysis of DGGE profiles revealed significant individual differences in the rats' intestinal microbiota.
作者：Zhao, Kai；Ren, Fangfang；Han, Fangting；Liu, Qiwen；Wu, Guogan；Xu, Yan；Zhang, Jian；Wu, Xiao；Wang, Jinbin；Li, Peng；Shi, Wei；Zhu, Hong；Lv, Jianjun；Zhao, Xiao；Tang, Xueming
作者单位：上海农业科学院
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2016
出版刊次：10
点击下载：通过基因水平转移、致敏性和肠道菌群来评价转基因水稻T1C-1对Sprague-Dawley大鼠的食用安全