中文摘要：本文的研究目的是评价转Cry1Ac基因抗虫甘蔗的生物安全问题。作者在120天的短期喂养试验中，将20只肉仔鸡随机关在四个笼子里。其中，I组饲喂商业鸡饲料；II组饲喂非转基因甘蔗饲料；III组饲喂转基因饲料（商业转基因比为1:1），IV组饲喂高剂量的转基因饲料（商业转基因比为1:2）。在试验过程中，四组小鸡的体重正常增加；四组小鸡血液的生化试验和分子检测没有显著差异。在实验结束时，小鸡被宰杀并解剖其重要器官用于组织学研究。研究发现，3组、4组的组织细胞与1组、2组的组织细胞在形态学和组织学上相似。另外，从鸡组织细胞中分离的总RNA中没有发现mRNA的表达。研究结果表明，转基因甘蔗对鸡的健康无有毒或有害的影响，且即使在鸡的重要组织中也没有发现从转基因作物转移到动物血液的转基因片段。因此，至少在短期喂养试验中，转基因甘蔗对其喂养的动物没有产生不利影响。
外文摘要：Genetically modified organisms have become obligatory to adopt certain precautions to overcome the risks related to release of GMOs. Through this study, an attempt was made to evaluate bio-safety concerns of transgenic sugarcane with insect resistant gene, Cry1Ac. In short term feeding trial of 120 days, 20 broiler chicks divided into four random groups were caged separately. Group-I fed on commercial chicken diet; group-II fed on non-transgenic sugarcane diet; group-III fed on transgenic diet (commercial to transgenic ratio was 1:1) while group-IV was fed on high dose transgenic diet (commercial to transgenic ratio was 1:2). Body weights of chicks increased normally in all four groups during the course of study. Biochemical tests and molecular tests done on chick's blood reported no significant difference among assorted groups. At the termination of the experiment, chicks were slaughtered and dissected vital organs were used for histological studies. It was found that tissues belong to group 3 and 4 were morphologically and histological similar to group 1 and 2. Also, no mRNA expression was observed in total RNA isolated from chicken's tissues. These findings suggest that there was no deleterious or harmful effect of the GM sugarcane on the chick's health and no transgene fragment has transferred from GM crop to animal's blood neither in their vital tissues. Conclusively, it is suggested that at least in short-term period feeding trial, the genetically modified sugarcane doesn't not have adverse effect on animals feeding them.
外文关键词：Bt Sugarcane；Feeding Trial；Broiler chicks；Biosafety assessment
作者：Hameed, A；Nasir, I. A；Tabassum, B；等
作者单位：Univ Punjab
期刊名称：JOURNAL OF ANIMAL AND PLANT SCIENCES
期刊影响因子：0.422
出版年份：2016
出版刊次：8
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