中文摘要：脱氧雪腐镰刀菌烯醇（DON）是二次真菌代谢物和污染物霉菌毒素，在世界各地种植的小麦和玉米产品中被广泛检测到。在过去二十年中，生物修复方法已经得到广泛研究，并有关于用该方法来降低DON相关毒性的报道。根据最近的报道，相比母本化合物DON而言，DON在C3的细菌差向异构作用使得其诱导物立体异构体（3-epi-DON）的生物毒性明显降低。作者在前期研究中，利用不同的哺乳动物细胞系和小鼠模型证实了3-epi-DON降低的生物效能，并从动力学上证实了其原因是在核糖体肽基转移酶中心（PTC）内3-epi-DON结合降低。在本研究中，作者检查了3-epi-DON的色谱行为以及其与典型酶（禾谷镰刀菌Tri101乙酰转移酶）的分子互作机制，为除了取消PTC结合外DON在C3的细菌差向异构作用对其分子互作机制的影响研究提供了证据。
外文摘要：Deoxynivalenol (DON) is a secondary fungal metabolite and contaminant mycotoxin that is widely detected in wheat and corn products cultivated around the world. Bio-remediation methods have been extensively studied in the past two decades and promising ways to reduce DON-associated toxicities have been reported. Bacterial epimerization of DON at the C3 carbon was recently reported to induce a significant loss in the bio-toxicity of the resulting stereoisomer (3-epi-DON) in comparison to the parental compound, DON. In an earlier study, we confirmed the diminished bio-potency of 3-epi-DON using different mammalian cell lines and mouse models and mechanistically attributed it to the reduced binding of 3-epi-DON within the ribosomal peptidyl transferase center (PTC). In the current study and by inspecting the chromatographic behavior of 3-epi-DON and its molecular interactions with a well-characterized enzyme, Fusarium graminearum Tri101 acetyltransferase, we provide the evidence that the C3 carbon epimerization of DON influences its molecular interactions beyond the abrogated PTC binding.
外文关键词：deoxynivalenol；epimer；polarity；Tri101；molecular；interactions
作者：Hassan，Yousef I.; Zhu, Honghui; Zhu, Yan; Zhou, Ting
作者单位：Agr & Agri Food Canada
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：9
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