中文摘要：本文介绍了金电极表面功能化策略，旨在获得一种用于检测黄曲霉毒素B1的新的抗阻测定免疫传感器。该免疫传感器的特点是通过四个步骤将黄曲霉毒素抗体固定在由牛血清白蛋白金连模修饰的金电极上。实验结果表明，该免疫传感器用于植物提取物中的黄曲霉毒素检测具有良好的回收率。
外文摘要：This paper describes the strategy of functionalization of gold electrodes surfaces in aim to obtain a new impedimetric immunosensor for the detection of aflatoxin B1, a common toxic food contaminant. The immunosensor elaboration is based on immobilization of anti-aflatoxin antibody on gold electrodes modified with a cross-linked film of bovine serum albumin, by a four-step protocol. The immunosensor is based on a simple design and requires small volumes of toxic aflatoxin solution. All the steps of the immunosensor elaboration and the immunochemical reaction between aflatoxin and antibody were followed using atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS). The resistance to charge transfer (Rct) was the most sensitive parameter to changes induced to the interfacial properties of the immunosensor by the incubation with aflatoxin and varied linearly with aflatoxin concentration in the range 1-20 ng/mL. The immunosensor was applied for the detection of aflatoxin in spiked plant extracts with good recovery factors.
外文关键词：surface functionalization；aflatoxin B1 detection；impedimetric immunosensor；electrochemical impedance spectroscopy
作者：Badea, Mihaela; Floroian, Laura; Restani, Patrizia; Moga, Marius
作者单位：Transilvania Univ Brasov,Romania
期刊名称：INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
期刊影响因子：1.692
出版年份：2016
出版刊次：8
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