中文摘要:本文旨在评估在0.1、1.0和10最大残留量值加标水平下,采用QuEChERS法定量检测金冠和红星苹果中四种农药残留(毒死蜱、乐果、茚虫威、吡虫啉)的效果。本文首先采用传统的QuEChERS法进行萃取和清理后,采用LC-MS/MS法进行色谱分析。根据t检验,两种基质中毒死蜱的基质效应均不显著,而乐果、茚虫威和吡虫啉的基质效应均显著,因此,本文采用基质匹配校准消除基质效应并采用MC进行定量检测。所有农药残留回收率为90.15%,相对标准差为13.27% (n = 330),检测限均低于最大残留值,此外,回收率、精密度、精确度以及线性关系等参数值均在所要求的范围之内。
外文摘要:This study was undertaken to validate the quick, easy, cheap, effective, rugged and safe (QuEChERS) method using Golden Delicious and Starking Delicious apple matrices spiked at 0.1 maximum residue limit (MRL), 1.0MRL and 10MRL levels of the four pesticides (chlorpyrifos, dimethoate, indoxacarb and imidacloprid). For the extraction and cleanup, original QuEChERS method was followed, then the samples were subjected to liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS) for chromatographic analyses. According to t test, matrix effect was not significant for chlorpyrifos in both sample matrices, but it was significant for dimethoate, indoxacarb and imidacloprid in both sample matrices. Thus, matrix-matched calibration (MC) was used to compensate matrix effect and quantifications were carried out by using MC. The overall recovery of the method was 90.15% with a relative standard deviation of 13.27% (n = 330). Estimated method detection limit of analytes blew the MRLs. Some other parameters of the method validation, such as recovery, precision, accuracy and linearity were found to be within the required ranges.
外文关键词:Liquid chromatograph-mass spectrometer (LC-MS, MS), matrix effect, pesticides, QuEChERS, validation
作者:Tiryaki, Osman
作者单位:Canakkale Onsekiz Mart Univ, Dept Plant Protect, Fac Agr, TR-17020 Canakkale, Turkey.
期刊名称:JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
期刊影响因子:1.247
出版年份:2016
出版刊次:10
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