中文摘要：黄曲霉毒素是一种高毒性次生代谢物，主要由寄生曲霉产生，它可以对大量的农产品造成污染，包括谷物、花生和田间作物。近年来，有研究表明，草药（如大西洋黄连木亚种Kurdica）会抑制微生物生长，并且这些草药对黄曲霉毒素生产也有特别的影响。本文旨在研究P. atlantica subsp. kurdica作为天然化合物抑制寄生曲霉生长以及抗黄曲霉毒素的功能。研究结果表明，P. atlantica subsp. kurdica不仅能抑制寄生曲霉的生长，还具有抗毒作用，并且还能够以剂量依赖方式有效的降低黄曲霉毒素生产。因此，这种草药提取物可能是一种潜在的、可用于医学或工业农业中的霉菌毒素抗剂。
外文摘要：Background: Aflatoxins are highly toxic secondary metabolites mainly produced by Aspergillus parasiticus. This species can contaminate a wide range of agricultural commodities, including cereals, peanuts, and crops in the field. In recent years, research on medicinal herbs, such as Pistacia atlantica subsp. kurdica, have led to reduced microbial growth, and these herbs also have a particular effect on the production of aflatoxins as carcinogenic compounds.
Objectives: In this study, we to examine P. atlantica subsp. kurdica as a natural compound used to inhibit the growth of A. parasiticus and to act as an anti-mycotoxin.
Materials and Methods: In vitro antifungal susceptibility testing of P. atlantica subsp. kurdica for A. parasiticus was performed according to CLSI document M38-A2. The rate of aflatoxin production was determined using the HPLC technique after exposure to different concentrations (62.5 - 125 mg/mL) of the gum. The changes in expression levels of the aflR gene were analyzed with a quantitative real-time PCR assay.
Results: The results showed that P. atlantica subsp. kurdica can inhibit A. parasiticus growth at a concentration of 125 mg/mL. HPLC results revealed a significant decrease in aflatoxin production with 125 mg/mL of P. atlantica subsp. kurdica, and AFL-B1 production was entirely inhibited. Based on quantitative real-time PCR results, the rate of aflR gene expression was significantly decreased after treatment with P. atlantica subsp. kurdica.
Conclusions: Pistacia atlantica subsp. kurdica has anti-toxic properties in addition to an inhibitory effect on A. parasiticus growth, and is able to decrease aflatoxin production effectively in a dose-dependent manner. Therefore, this herbal extract maybe considered a potential anti-mycotoxin agent in medicine or industrial agriculture.
外文关键词：HPLC;Real-Time PCR;Aflatoxin;Aspergillus parasiticus;Pistacia atlantica subsp Kurdica
作者：Khodavaisy, Sadegh; Rezaie, Sassan; Noorbakhsh, Fatemeh; Baghdadi, Elham; Sharifynia, Somayeh; Aala, Farzad
作者单位：Kurdistan Univ Med Sci,Iran.
期刊名称：JUNDISHAPUR JOURNAL OF MICROBIOLOGY
期刊影响因子：0.655
出版年份：2016
出版刊次：7
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