中文摘要：转BT基因植物的广泛种植可为社会带来经济效益；然而其造成的潜在风险越来越受到人们的重视。在连续三年时间内，作者采用酶联免疫吸附测定法和荧光定量PCR（RT-PCR）检测了741棵6-8岁的转基因抗虫杨树中Bt毒蛋白的时间和空间动态。本文调查了Bt毒蛋白的富集、分布和降解，及转基因杨树对靶标昆虫种群美国白蛾的影响。研究发现，在转基因杨树中Cry1Ac毒蛋白的含量呈动态变化，且在八月达到最大值；Cry1Ac基因的mRNA转录和Cry1Ac毒蛋白的水平基本一致，但mRNA转录在七月达到最大值。在转基因杨树林的土壤中可检测到Cry1Ac毒蛋白的微观水平，且未随转基因杨树的种植年份而增加积累量。在转基因杨树林实验中，对白蛾密度的系统调查表明，转基因杨树Pb29能在一定程度抵抗害虫；且在靶标害虫出现高峰期，转基因杨树林的白蛾密度要显著低于非转基因杨树林的白蛾密度。
外文摘要：Extensive planting of Bacillus thuringiensis (Bt)-transgenic plants economically benefits society; however, the potential risk they pose is receiving increasing attention. This study used enzyme-linked immunosorbent assay and fluorescence quantitative PCR (RT-PCR) to monitor the temporal and spatial dynamics of the expression of Bt toxic protein in a forest of 6- to 8-year-old trees of transgenic insect-resistant poplar 741 for three consecutive years. The enrichment, distribution, and degradation of Bt toxic protein and the influence of transgenic poplars on the targeted insect population, Hyphantria cunea, were investigated. The content of Cry1Ac toxic protein dynamically changed in transgenic poplar. During the annual growth cycle, the content initially increased, then decreased in the long and the short branches of the crown and in the root system, peaking in August. During the study, the protein did not accumulate overtime. The mRNA transcription of gene Cry1Ac was almost consistent with the level of the protein, but transcription peaked in July. In the transgenic and control forestland, microscale levels of the Cry1Ac toxic protein were detected from the soil, but increased accumulation was not observed with the planting year of transgenic poplar. Meanwhile, Bt was isolated and detected molecularly from the soil in the experimental forestland. A systematic investigation of the density of H. cunea in the experimental transgenic poplar forest indicated that transgenic Pb29 poplar could resist insects to a certain degree. At peak occurrence of the targeted insects, the density of H. cunea in the experimental forest was significantly lower than in the nontransgenic poplar forest.
外文关键词：Biosecurity；Bt toxic protein；Targeted insect；Transgenic insect-resistance poplar
作者：Zhang, Yiwen；Zhang, Jun；Lan, Jinping；等
作者单位：河北农业大学
期刊名称：JOURNAL OF FORESTRY RESEARCH
期刊影响因子：0.929
出版年份：2016
出版刊次：12
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