中文摘要：本研究测试了从巴西亚马逊地区水环境中分离的芽孢杆菌对霉菌毒素产毒真菌的抗性。所有受试菌均显示抑菌活性，至少能抑制7种指示菌。随后筛选出4株抑菌能力强的杆菌，评估其在减少真菌菌丝生长速率、孢子形成、孢子萌发及霉菌毒素产生方面的活性。结果发现它们能显著影响产毒真菌，是很好的抑制产毒真菌的生物防治剂。
外文摘要：Bacillus strains isolated from the aquatic environment of the Brazilian Amazon region were tested for their activity against mycotoxigenic fungi. All tested bacteria showed antifungal activity, inhibiting at least 7 indicator fungi. Four Bacillus strains showing promising antifungal results were subsequently evaluated for their activity in reducing mycelial growth rate, sporulation, spore germination percentage, and mycotoxin production. Bacillus sp. P1 and Bacillus sp. P11 had a remarkable antifungal effect on toxigenic fungi. Washed bacterial cell suspension of strains P1 and P11 (107 CFU/ml) reduced by > 70% the fungal colony diameters, including a complete inhibition of ochratoxin A (OTA) producing Aspergillus spp. Significant reduction of growth rate, sporulation and spore germination were also observed. The bacteria influenced the production of mycotoxins, causing a reduction around 99 and 97% in AFB1 and OTA concentration, respectively. Chromatographic analysis revealed the presence of lipopeptides (iturin A and surfactin isomers) in butanol extracts of cell-free supernatants and cell pellets of strains P1 and P11. Furthermore, antifungal activity of these extracts was confirmed against A. flavus A12 and A. carbonarius ITAL293, producers of AFB1 and OTA, respectively. These bacterial strains could be promising biocontrol agents against toxigenic fungi.
外文关键词：Bacillus species；Biocontrol；Iturin；Mycotoxins；Surfactin
作者：Veras, FF；Correa, APF；Welke, JE；Brandelli, A
作者单位：Univ Fed Rio Grande do Sul
期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
期刊影响因子：3.445
出版年份：2016
出版刊次：12
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