中文摘要：黄曲霉毒素B1(AFB1)是一种黄曲霉和寄生曲霉产生的剧毒性黄曲霉毒素，受它污染的谷物和其它农作物，会给人类健康造成严重威胁，是一个严重的食品安全问题。本研究中，开发了一种竞争性的基于上转换荧光技术的侧流分析法(AFB1-UPT-LF)，用于检测AFB1。优化AFB1提取方法后，该分析法对各种作物样品均具有良好的耐受性，其检测限（0.1到5 ng /g）低于我国规定的相应的最高残留限量（MRL）。因此，AFB1-UPT-LF分析法因其高灵敏性、特异性及样品耐受性，为现场快速检测AFB1提供了有效的工具。
外文摘要：Contamination of grains and other crops by aflatoxin B1 (AFB1), a highly toxic aflatoxin produced by Aspergillus flavus and Aspergillus parasiticus, poses a serious threat to human health and is an important food safety issue. In this study, a competitive up-converting phosphor technology-based lateral flow (AFB1-UPT-LF) assay was developed for rapid detection of AFB1. Detection sensitivity of the proposed assay can reach 0.03 ng mL−1 for standard AFB1 solutions, with the coefficients of variation (CV) less than 10% (from 1.0 to 9.4%). A good linearity (r=0.9889) was observed for quantification of AFB1 from 0.03 to 1000 ng mL−1. Except for aflatoxin M1, no cross-reactivity was found with the abrin, ricin, ochratoxin A, botulinum toxin, shiga toxin 1, shiga toxin 2, and staphylococcal enterotoxin B, even at high concentrations of 100 or 1000 ng mL−1. After optimizing the extraction of AFB1, the assay showed good tolerance to various crop samples, with the detection limit (from 0.1 to 5 ng g−1) lower than the corresponding maximum residue level (MRL) set in China. The AFB1-UPT-LF assay provides a promising tool for rapid on-site detection of AFB1 because of its high sensitivity, specificity, and sample tolerance.
外文关键词：Up-converting phosphor；Lateral flow assay；Aflatoxin B1；On-site detection
作者：Zhao, Y；Liu, X；Wang, XC；Sun, CY；Wang, XR；Zhang, PP；Qiu, JF；Yang, RF；Zhou, L
作者单位：Beijing Inst Microbiol & Epidemiol
期刊名称：TALANTA
期刊影响因子：4.035
出版年份：2016
出版刊次：12
点击下载：一种基于上转换荧光技术的侧流分析法的开发和评价，可用于快速定量检测农作物中的黄曲霉毒素B1