中文摘要：本研究开发了一种综合利用印迹技术和磁石制作磁性分子印迹搅拌棒(MMIP-SB)的快捷、简便的方法，并用MMIP-SB来检测黄曲霉毒素。MMIP-SB可作为常用搅拌棒，与高效液相色谱-质谱联用分析法结合，用于检测牛奶中的黄曲霉毒素M1及婴儿谷类辅食中的黄曲霉毒素B1、B2、G1和G2。本研究制备的搅拌棒具有良好的搅拌、提取性能及识别能力，能替代其它更加复杂的制作工艺。
外文摘要：A fast and facile method for the fabrication of magnetic molecularly imprinted stir-bars (MMIP-SB) has been developed, using a combination of imprinting technology and magnetite. Magnetite was prepared in the laboratory from the raw and embedded into molecularly imprinted polymers through a process of bulk polymerization. This novel design was applied to the analysis of aflatoxins, one of the most important groups of mycotoxins in terms of occurrence and toxicity. In the context of food safety, molecularly imprinted polymers are a promising tool to achieve selective and accessible methods of extraction for different residues and contaminants. Considering the toxicity of aflatoxins, a dummy template was preferred for the synthesis of the imprinted polymers. A rapid and affordable extraction method for isolating five different aflatoxins that may be present in food was developed. The MMIP-SB was used as a conventional stir-bar and combined with high performance liquid chromatography and mass spectrometry for the determination of aflatoxin M1 in milk powder (infant formulas) and aflatoxins B1, B2, G1 and G2 in cereal-based baby foods. The results showed an average recovery of 60%, 43, 40, 44 and 39%, respectively, and RSD below 10%. These in-house prepared stir-bars featured good stirring and extraction performance, and recognition abilities, offering a good alternative to more complicated.
外文关键词：Aflatoxin；MIP；Stir-bar；HPLC-MS/MS；Milk；Cereal
作者：Diaz-Bao, M；Regal, P；Barreiro, R；Fente, CA；Cepeda, A
作者单位：Univ Santiago de Compostela
期刊名称：JOURNAL OF CHROMATOGRAPHY A
期刊影响因子：3.926
出版年份：2016
出版刊次：11
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