中文摘要：本文集成了一个能同时检测两种甲氧基丙烯酸酯类杀菌剂（醚菌酯和肟菌酯）的间接酶联免疫吸附试验(ELISA)。实验结果显示，本文制备的抗血清对醚菌酯和肟菌酯具有高亲和力和高敏感性，且他们对醚菌酯的抑制剂浓度和检测限分别为14.7μg/ml- 0.0044μg/ml，对肟菌酯的抑制剂浓度和检测限分别为22.9μg/ml -0.014μg/ml，对其他甲氧基丙烯酸酯类杀菌剂的交叉反应速率低于0.1%，且在100μg/ml 、10μg/ml 和1μg/ml加标水平下，回收率范围为82-104%。
外文摘要：To assemble an indirect competitive enzyme-linked immunosorbent assay (ELISA) for estimating two strobilurin fungicides at the same time, the hapten was synthesised which contained the common active group, (E)-2-(2-bromophenyl)-2-(methoxyimino) acetic acid methyl ester (OEBr) in kresoxim-methyl and trifloxystrobin. The immunogen and coating antigen were respectively prepared through conjugating the above-mentioned hapten with BSA and OVA by the mixed anhydride and activated ester methods, and polyclonal antibodies were produced by immunised rabbits. An enzyme-linked immunosorbent assay was developed for simultaneous detection of kresoxim-methyl and trifloxystrobin. In ELISA, the antiserum showed high affinity and sensitivity to kresoxim-methyl and trifloxystrobin, and their IC50 value and detection limit (expressed as IC10) were 14.7 and 0.0044 mu g/ml, respectively, for kresoxim-methyl, and 22.9 and 0.014 mu g/ml, respectively, for trifloxystrobin. The cross-reaction rate was below 0.1% for other strobilurin fungicides. Recovery study of ELISA from spiked samples of homogenised peeled oranges (final concentrations of 100, 10, and 1 mu g/ml) resulted in recovery levels in the range of 82-104%.
 
外文关键词：kresoxim-methyl, trifloxystrobin, polyclonal antibody, ELISA, recovery rate
作者：Yang, Wei; Zhu, Jie; Liu, Ming-Qi; Dai, Xian-Jun
作者单位： China Jiliang Univ, Coll Life Sci, Hangzhou 310018, Zhejiang, Peoples R China.
期刊名称：CZECH JOURNAL OF FOOD SCIENCES,
期刊影响因子：0.728
出版年份：2016
出版刊次：5
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