中文摘要：本文建立了一种可靠、简单、快速、廉价且稳健的样本预处理方法，该方法可用于紫外检测液相色谱法（HPLC）检测酒酿葡萄中细交链孢菌酮酸残留。该方法基于改进QuEChERS法采用乙酸乙酯做萃取溶剂，最优条件下定量限达到0.05μg/g，在0.05μg/g、0.5μg/g和5μg/g加标水平下，葡萄样本总回收率分别为96%、82%和97%，且相对标准差小于8%，霍维茨比率小于0.11。本文还应用该方法对葡萄样本中0.057-0.595μg/g浓度范围的细交链孢菌酮酸残留进行了定量检测。该方法适用于葡萄酒产业中高产量葡萄常规质量控制，亦可应用于一般实验室。
外文摘要：A reliable, simple, fast, inexpensive and robust sample preparation approach for tenuazonic acid (TA) determination in grapes by liquid chromatography with ultraviolet detection (HPLC-UV) is proposed. The method is based on a modified QuEChERS (quick, easy, cheap, effective, rugged and safe) approach using ethyl acetate as extraction solvent. Its involves extraction of 2.5 g sample (plus 2.5 mL 1% formic acid acidified water) with 5 mL of ethyl acetate; drying/cleaning- up of extract with 0.25 g anhydrous CaCl2 and determination by HPLC-UV. The method was optimized and validated achieving a limit of quantification (LOQ) of 0.05 mu g g(-1). The overall recoveries were 96%, 82% and 97% for grape samples spiked at 0.05, 0.5 and 5 mu g g(-1), respectively. The method showed excellent reproducibility with RSDs for the above data <= 8% and Horwitz ratios <0.11. The procedure was applied to evaluate the occurrence of TA in grapes and it was quantified at concentrations ranging between 0.057 and 0.595 mu g g(-1). The methodological development allows a high throughput routine quality control of grapes, adding a valuable tool for the winemaking industry. The method could be applied in an average laboratory and help to understand possible effects related to Alternaria rotting on the final wines elaborated. As well, this is the first report of TA presence in grapes used for wine elaboration, so the results add new knowledge to a growing research area.
外文关键词：PERFORMANCE LIQUID-CHROMATOGRAPHY; SOLID-PHASE EXTRACTION; ALTERNARIA TOXINS; MASS-SPECTROMETRY; TOMATO PRODUCTS; HPLC-MS/MS; SAMPLES; FRUITS
作者：Fontana, Ariel R.; Prendes, Luciana P.; Morata, Vilma I.; Bottini, Ruben
作者单位：Univ Nacl Cuyo, Consejo Nacl Invest Cient & Tecn, Inst Biol Agr Mendoza, Lab Bioquim Vegetal, Almirante Brown 500,M5528AHB Chacras de Coria, Mendoza, Argentina.
期刊名称：RSC ADVANCES
期刊影响因子：3.289
出版年份：2016
出版刊次：8
点击下载：应用高通量修饰QuEChERS法检测酒酿葡萄中霉菌毒素细交链孢菌酮酸残留