中文摘要：本文建立了一种有效检测烟草中55种农药残留的方法并进行了实证研究。该方法采用乙腈、低温沉淀和分散固相萃取净化法进行样本预处理，采用气相色谱-串联质谱法结合超高效液相色谱-串联质谱法进行定量检测。该检测方法的定量限在25μg/kg-75μg/kg之间，在25μg/kg、75μg/kg、125μg/kg和250μg/kg加标水平下，回收率范围为63%-161%，相对标准差小于等于20%。应用该方法成功地对13种烟草样本进行了常规残留检测，其甲基嘧啶磷和异柳磷农药残留范围为35μg/kg-51μg/kg。
外文摘要：An effective method has been developed and validated for the determination of residues of 55 pesticides in tobacco. The proposed sample preparation method is based on acetonitrile extraction, low-temperature precipitation (LTP) and dispersive solid phase extraction (d-SPE) clean-up. Gas chromatography and ultra-high performance liquid chromatography analysis, both coupled to tandem mass spectrometry (GC-MS/MS and UHPLC-MS/MS), were used for determination. LTP is easy to perform and was crucial to obtain a clean extract. Method quantification limit for the pesticides were between 25 and 75 mu g kg(-1). Extraction recoveries obtained for blank samples spiked at 25, 75, 125 and 250 mu g kg(-1) levels ranged from 63 to 161% with relative standard deviations (RSD) <= 20%. The method was successfully applied to the analysis of thirteen different tobacco samples, providing to be a robust procedure for routine analysis. The compounds pirimiphos methyl and isofenphos presented residues in the range of 35-51 mu g kg(-1). (C) 2016 Elsevier B.V. All rights reserved.
 
外文关键词：Sample preparation, Pesticides, Tobacco, Chromatography
作者：Bernardi, Gabrieli; Kemmerich, Magali; Ribeiro, Lucila C.; Adaime, Martha B.; Zanella, Renato; Prestes, Osmar D.
作者单位：Univ Fed Santa Maria, Dept Chem, Lab Pesticide Residue Anal LARP, BR-97105900 Santa Maria, RS, Brazil.
期刊名称：TALANTA
期刊影响因子：4.035
出版年份：2016
出版刊次：8
点击下载：一种采用GC-MS/MS和UHPLC-MS/MS技术检测烟草中农药残留的方法